An adaptive ERG technique to measure normal and altered dark adaptation in the mouse
| Autor: | Y. Katagiri, Henry J. Kaplan, Maureen A. McCall, Volker Enzmann, Paul J. DeMarco |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
genetic structures Mutant Iodates Functional response Dark Adaptation Biology Stimulus (physiology) Retina Mice chemistry.chemical_compound Optics Retinal Diseases Retinal Rod Photoreceptor Cells Physiology (medical) Electroretinography medicine Animals Sodium iodate Retinal pigment epithelium medicine.diagnostic_test business.industry Retinal Mice Mutant Strains Sensory Systems Mice Inbred C57BL Ophthalmology medicine.anatomical_structure chemistry Retinal Cone Photoreceptor Cells Biophysics sense organs business |
| Zdroj: | DeMarco, Paul J; Katagiri, Yoshiaki; Enzmann, Volker; Kaplan, Henry J; McCall, Maureen A (2007). An adaptive ERG technique to measure normal and altered dark adaptation in the mouse. Documenta ophthalmologica, 115(3), pp. 155-63. Dordrecht: Springer 10.1007/s10633-007-9078-5 |
| ISSN: | 1573-2622 0012-4486 |
| DOI: | 10.1007/s10633-007-9078-5 |
| Popis: | The time-course of dark adaptation provides valuable insights into the function and interactions between the rod and cone pathways in the retina. Here we describe a technique that uses the flash electroretinogram (ERG) response to probe the functional integrity of the cone and rod pathways during the dynamic process of dark adaptation in the mouse. Retinal sensitivity was estimated from the stimulus intensity required to maintain a 30 μV criterion b-wave response during a 40 min period of dark adaptation. When tracked in this manner, dark adaptation functions in WT mice depended upon the bleaching effects of initial background adaptation conditions. Altered dark adaptation functions, commensurate with the functional deficit were recorded in pigmented mice that lacked cone function (Gnat2 cplf3 ) and in WT mice injected with a toxin, sodium iodate (NaIO3), which targets the retinal pigment epithelium and also has downstream effects on photoreceptors. These data demonstrate that this adaptive tracking procedure measures retinal sensitivity and the contributions of the rod and/or cone pathways during dark adaptation in both WT control and mutant mice. |
| Databáze: | OpenAIRE |
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