Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro
| Autor: | Luis O. Burzio, Verónica A. Burzio, Nicole Sanhueza, Nicole Farfán, Macarena Briones |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
RNA Mitochondrial Short Report Biology law.invention lncRNA law Cell Line Tumor microRNA RNA Antisense Biology (General) Gene miRNA Cancer Cell Proliferation Gene knockdown RNA Non-coding RNA ncRNA In vitro Cell biology Gene Expression Regulation Neoplastic MicroRNAs biology.protein Suppressor RNA Long Noncoding Dicer Cloning |
| Zdroj: | Biological Research Biological Research, Vol 54, Iss 1, Pp 1-7 (2021) |
| ISSN: | 0717-6287 0716-9760 |
| Popis: | Background The antisense noncoding mitochondrial RNAs (ASncmtRNAs) derive from the mitochondrial 16S gene. Knockdown of these transcripts with chemically-modified antisense oligonucleotides induces proliferative arrest, apoptosis and invasiveness reduction in tumor but not normal cells. One of these transcripts, ASncmtRNA-2, contains the complete and identical sequence of hsa-miR-4485-3p and, upon knockdown of this transcript, there is a strong increase in levels of this miRNA, suggesting ASncmtRNA-2 as a source for miR-4485-3p, which is supported by several evidences from our group and others, in the ex vivo setting. Results Here we show that incubation of in vitro-transcribed ASncmtRNA-2 with recombinant Dicer produces RNA fragments corresponding to hsa-miR-4485-3p, showing that Dicer binds to and processes ASncmtRNA-2, strongly supporting the hypothesis that ASncmtRNA-2 acts as a precursor for miR-4485-3p. Conclusion The in vitro results presented here strengthen the hypothesis that miR-4485-3p is derived from ASncmtRNA-2 by Dicer processing. Since miR-4485-3p is classified as a tumor suppressor miRNA, this evidence strengthens the application of ASncmtRNA knockdown for cancer therapy. |
| Databáze: | OpenAIRE |
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