Effect of Butyrate on Collagen Expression, Cell Viability, Cell Cycle Progression and Related Proteins Expression of MG-63 Osteoblastic Cells
| Autor: | Sin-Yuet Yeung, Mei-Chi Chang, Chiu-Po Chan, Hsin-Cheng Liu, Chia-Mei Tang, Jiiang-Huei Jeng, Yi-Ling Tsai, Ming-Wei Liao, Eric J. W. Liou, Tong-Mei Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Protein Expression Carboxylic Acids lcsh:Medicine Biochemistry Butyric acid chemistry.chemical_compound 0302 clinical medicine Spectrum Analysis Techniques Animal Cells Bone cell Medicine and Health Sciences Cell Cycle and Cell Division lcsh:Science Connective Tissue Cells Multidisciplinary Organic Compounds Cell Cycle Flow Cytometry Chemistry medicine.anatomical_structure Cell Processes Connective Tissue Spectrophotometry Physical Sciences Cytophotometry Cellular Types Anatomy Type I collagen Research Article Cyclin-Dependent Kinase Inhibitor p21 Cell Survival Butyric Acids Butyrate Biology Research and Analysis Methods Collagen Type I Cell Line 03 medical and health sciences Cyclins medicine Gene Expression and Vector Techniques Humans Viability assay Cyclin B1 Molecular Biology Techniques Molecular Biology Cell Cycle Inhibitors Cell Proliferation Molecular Biology Assays and Analysis Techniques Osteoblasts Cell growth lcsh:R Organic Chemistry Chemical Compounds Biology and Life Sciences Proteins 030206 dentistry Cell Biology Molecular biology 030104 developmental biology Biological Tissue chemistry Gene Expression Regulation Cell culture Butyric Acid lcsh:Q Reactive Oxygen Species Collagens Acids |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 11, p e0165438 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Aims Butyric acid is one major metabolic product generated by anaerobic Gram-negative bacteria of periodontal and root canal infection. Butyric acid affects the activity of periodontal cells such as osteoblasts. The purposes of this study were to investigate the effects of butyrate on MG-63 osteoblasts. Methods MG-63 cells were exposed to butyrate and cell viability was estimated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The mRNA and protein expression of type I collagen and cell cycle-related proteins were measured by reverse-transcriptase polymerase chain reaction (RT-PCR), western blotting or immunofluorescent staining. Cellular production of reactive oxygen species (ROS) was analyzed by 2',7'-dichlorofluorescein (DCF) fluorescence flow cytometry. Results Exposure to butyrate suppressed cell proliferation, and induced G2/M (8 and 16 mM) cell cycle arrest of MG-63 cells. Some cell apoptosis was noted. The mRNA expression of cdc2 and cyclin-B1 decreased after exposure to butyrate. The protein expression of type I collagen, cdc2 and cyclin B1 were decreased, whereas the expression of p21, p27 and p57 was stimulated. Under the treatment of butyrate, ROS production in MG-63 cells markedly increased. Conclusions The secretion of butyric acid by periodontal and root canal microorganisms may inhibit bone cell growth and matrix turnover. This is possibly due to induction of cell cycle arrest and ROS generation and inhibition of collagen expression. These results suggest the involvement of butyric acid in the pathogenesis of periodontal and periapical tissue destruction by impairing bone healing responses. |
| Databáze: | OpenAIRE |
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