Epidemiology of carbapenem-resistant Escherichia coli and first report of blaVIM carbapenemases gene in calves from India

Autor: Prasanna Vadhana, D.K. Sinha, B.S. Pruthvishree, B.R. Singh, Ajay Yadav, M Senthil Murugan, K. R. Nirupama, O.R. Vinodh Kumar, Monika Bhardwaj
Rok vydání: 2019
Předmět:
Diarrhea
0301 basic medicine
Carbapenem
Imipenem
Genotype
040301 veterinary sciences
Epidemiology
030106 microbiology
carbapenem resistance
Cattle Diseases
India
Biology
medicine.disease_cause
Polymerase Chain Reaction
Meropenem
beta-Lactam Resistance
beta-Lactamases
Microbiology
0403 veterinary science
03 medical and health sciences
chemistry.chemical_compound
Plasmid
Bacterial Proteins
Escherichia coli
polycyclic compounds
medicine
Animals
Escherichia coli Infections
Original Paper
E. coli
04 agricultural and veterinary sciences
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Calf
Carbapenem-Resistant Enterobacteriaceae
Cross-Sectional Studies
Infectious Diseases
chemistry
Carrier State
bacteria
Multilocus sequence typing
Cattle
Efflux
Ertapenem
Multilocus Sequence Typing
medicine.drug
Zdroj: Epidemiology and Infection
ISSN: 1469-4409
0950-2688
DOI: 10.1017/s0950268819000463
Popis: A cross-sectional study on six dairy farms was conducted to ascertain the occurrence of carbapenem-resistant Escherichia coli in calves. Two-hundred and seventy-nine isolates of E. coli were recovered from 90 faecal samples from apparently healthy (45) and diarrhoeal (45) calves. The isolates were screened for phenotypic susceptibility to carbapenems and production of metallo β-lactamase, as well as five carbapenemase resistance genes by PCR, and overexpression of efflux pumps. Eighty-one isolates (29.03%) were resistant to at least one of three carbapenem antibiotics [meropenem (23.30%), imipenem (2.15%) and ertapenem (1.43%)], and one isolate was positive for the blaVIM gene which was located on an Incl1 plasmid of a novel sequence type (ST 297) by multilocus sequence typing. The majority (83.95%) of isolates had an active efflux pump. Calves housed on concrete floors were approximately seven times more likely to acquire meropenem-resistant isolates than those housed on earthen floors (95% CI 1.27–41.54). In India, carbapenem drugs are not used in food animal treatment, hence carbapenem-resistant strains in calves possibly originate from the natural environment or human contact and is of public health importance. To our knowledge, this is the first report of blaVIM carbapenemases gene in calves from India.
Databáze: OpenAIRE
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