Fusarium oxysporum as a Multihost Model for the Genetic Dissection of Fungal Virulence in Plants and Mammals
| Autor: | Josep Guarro, Marta P. Madrid, Emilio Mayayo, Montserrat Ortoneda, M. Isabel G. Roncero, Antonio Di Pietro, Zaira Caracuel |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Fusarium Genes Fungal Immunology Virulence Human pathogen Models Biological Microbiology Immunocompromised Host Mice Solanum lycopersicum Species Specificity Fusarium oxysporum Animals Humans Gene Plant Diseases Wilt disease Chitin Synthase biology fungi food and beverages Fungi imperfecti Chitin synthase biology.organism_classification Infectious Diseases Mycoses Mutation biology.protein Parasitology Mitogen-Activated Protein Kinases Fungal and Parasitic Infections Transcription Factors |
| Zdroj: | Infection and Immunity. 72:1760-1766 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Fungal pathogens cause disease in plant and animal hosts. The extent to which infection mechanisms are conserved between both classes of hosts is unknown. We present a dual plant-animal infection system based on a single strain of Fusarium oxysporum , the causal agent of vascular wilt disease in plants and an emerging opportunistic human pathogen. Injection of microconidia of a well-characterized tomato pathogenic isolate (isolate 4287) into the lateral tail vein of immunodepressed mice resulted in disseminated infection of multiple organs and death of the animals. Knockout mutants in genes encoding a mitogen-activated protein kinase, a pH response transcription factor, or a class V chitin synthase previously shown to be implicated in virulence on tomato plants were tested in the mouse model. The results indicate that some of these virulence factors play functionally distinct roles during the infection of tomato plants and mice. Thus, a single F. oxysporum strain can be used to study fungal virulence mechanisms in plant and mammalian pathogenesis. |
| Databáze: | OpenAIRE |
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