Influence of melatonin on IL-1Ra gene and IL-1 expression in rats with liver ischemia reperfusion injury
| Autor: | Qing Xu, Lei Gu, Ye Liu, Chunhui Jiang, Longci Sun, Hong Zhou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pathology medicine.drug_class medicine.medical_treatment Intraperitoneal injection Ischemia interleukin-1 receptor antagonist gene melatonin 030204 cardiovascular system & hematology Biology General Biochemistry Genetics and Molecular Biology Melatonin 03 medical and health sciences 0302 clinical medicine Internal medicine Gene expression medicine General Pharmacology Toxicology and Pharmaceutics ischemia reperfusion injury General Neuroscience Interleukin General Medicine Articles medicine.disease Receptor antagonist 030104 developmental biology Endocrinology Apoptosis Reperfusion injury interleukin-1 medicine.drug |
| Zdroj: | Biomedical Reports |
| ISSN: | 2049-9442 2049-9434 |
| Popis: | The aim of the present study was to explore the influence of melatonin (MT) on rats with liver ischemia reperfusion injury (IRI) and its mechanism. A total of 66 male Sprague-Dawley rats were randomly divided into 3 groups: i) Normal control group, ii) ischemia reperfusion group (IR group) and iii) melatonin treatment group (MT group). Rats in the MT group were administered an intraperitoneal injection of MT (10 mg/kg, 1 ml) at 70 and 35 min before ischemia, early reperfusion, and 1 and 2 h after reperfusion, respectively. Blood was removed at the normal time-point (prior to any processes), 35 min before ischemia, 2, 4, 8 and 24 h after reperfusion. Subsequently the rats were sacrificed. The pathological changes of liver tissues, interleukin-1 receptor antagonist (IL-1Ra) gene and IL-1 expression levels were detected. There were no evident differences between the immediate reperfusion and 2 h IR group and MT group. The liver structure injury of the 4, 8 and 24 h MT groups were improved to various differences compared to the corresponding IR group; liver IL-1β of the MT group at 35 min after ischemia, and 2, 4, 8 and 24 h after reperfusion was evidently lower than that of the IR group (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|