Identification of Differently Regulated Proteins after Fusarium graminearum Infection of Emmer (Triticum dicoccum) at Several Grain Ripening Stages
| Autor: | Katrin Paffenholz, Christina Trümper, Hans-Peter Braun, Petr Karlovsky, Inga Smit, Elke Pawelzik, Philip Kössler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Fusarium
Globulin plant-material General Chemical Engineering lcsh:Biotechnology culmorum Industrial and Manufacturing Engineering resistance ddc:570 mycotoxins Heat shock protein lcsh:TP248.13-248.65 expression Botany fusarium head blight grain development 2. Zero hunger biology lcsh:TP368-456 polyamine biosynthesis plant proteomics food and beverages Ripening head blight families biology.organism_classification defence proteins hordeum-vulgare lcsh:Food processing and manufacture Fusarium head blight host Chitinase Proteome ddc:660 biology.protein emmer Hordeum vulgare Spermidine synthase Food Science Biotechnology |
| Zdroj: | Food Technology and Biotechnology, Vol 53, Iss 3, Pp 261-268 (2015) ResearcherID Food Technology and Biotechnology 53 (2015), Nr. 3 |
| ISSN: | 1334-2606 1330-9862 |
| Popis: | This study was conducted to improve the knowledge of molecular processes involved in the interaction between Fusarium graminearum and emmer in the course of grain ripening. Emmer plants were artificially inoculated with a F. graminearum spore suspension at anthesis. In the course of grain ripening from milk ripe to plant death stage, grains at four phenological growth stages were collected for analysis. The infection degree was evaluated based on the F. graminearum DNA content in emmer grain infolding tissues (glumes and rachis). For proteome analysis the albumin and globulin fractions of emmer grains, consisting of proteins with various functions related to the development and stress response, were analysed regarding the changes due to Fusarium infection by two-dimensional gel electrophoresis. Altogether, forty-three proteins affected by infection were identified by mass spectrometry. Enzymes detoxifying reactive oxygen species were regulated at all developmental stages. In the early stage of grain development, the abundance of proteins related to stress response, such as 2-Cys peroxiredoxin, a chitinase, a xylanase inhibitor and a spermidine synthase was increased. During later stage of grain development, the abundance of stress-related proteins, such as chitinases, heat shock proteins and an alpha-amylase inhibitor-like protein, decreased. During all ripening stages, but especially during medium milk stage (BBCH 75) and soft dough stage (BBCH 85), the abundance of proteins related to carbon metabolism, starch and protein biosynthesis as well as photosynthesis increased due to F. graminearum infection. At the plant death stage (BBCH 97) the abundance of only two proteins related to metabolism decreased. |
| Databáze: | OpenAIRE |
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