Screening for antifungal, DNA-damaging and anticholinesterasic activities of Brazilian plants from the Atlantic Rainforest - Ilha do Cardoso State Park
| Autor: | Vanderlan da Silva Bolzani, Sonia M. C. Dietrich, Débora Gomes Agripino, Elaine Monteiro Cardoso Lopes, Rosana Cristina Carreira, Luce Maria Brandão Torres, Maria Claudia Marx Young, Inês Cordeiro |
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| Přispěvatelé: | Instituto de Botânica, Universidade Estadual Paulista (Unesp) |
| Předmět: |
Antifungal
medicine.drug_class lcsh:RS1-441 Rainforest Biology lcsh:Pharmacy and materia medica chemistry.chemical_compound Botany medicine Atlantic forest General Pharmacology Toxicology and Pharmaceutics Cladosporium sphaerospermum antifungal activity plant screening biology.organism_classification triagem de plantas chemistry acetylcholinesterase inhibitors Quantitative assay Atlantic Rainforest Plant species Floresta Mata Atlântica Sloanea guianensis atividade antifúngica inibidores da acetilcolinesterase DNA |
| Zdroj: | ResearcherID Revista Brasileira de Farmacognosia, Vol 18, Iss suppl, Pp 655-660 Revista Brasileira de Farmacognosia, Volume: 18 Supplement, Pages: 655-660, Published: DEC 2008 Revista Brasileira de Farmacognosia v.18 suppl.0 2008 Revista Brasileira de Farmacognosia Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0102-695X |
| Popis: | Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:57:45Z No. of bitstreams: 1 S0102-695X2008000500002.pdf: 197018 bytes, checksum: 4d0e47201afc7dde96c9fa15d2c21201 (MD5) Made available in DSpace on 2013-08-22T18:57:45Z (GMT). No. of bitstreams: 1 S0102-695X2008000500002.pdf: 197018 bytes, checksum: 4d0e47201afc7dde96c9fa15d2c21201 (MD5) Previous issue date: 2008-12-01 Made available in DSpace on 2013-09-30T19:52:50Z (GMT). No. of bitstreams: 2 S0102-695X2008000500002.pdf: 197018 bytes, checksum: 4d0e47201afc7dde96c9fa15d2c21201 (MD5) S0102-695X2008000500002.pdf.txt: 31110 bytes, checksum: 1781a0ec1a98b12664c1dba22c8ea1d2 (MD5) Previous issue date: 2008-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:20:51Z No. of bitstreams: 2 S0102-695X2008000500002.pdf: 197018 bytes, checksum: 4d0e47201afc7dde96c9fa15d2c21201 (MD5) S0102-695X2008000500002.pdf.txt: 31110 bytes, checksum: 1781a0ec1a98b12664c1dba22c8ea1d2 (MD5) Made available in DSpace on 2014-05-20T14:20:51Z (GMT). No. of bitstreams: 2 S0102-695X2008000500002.pdf: 197018 bytes, checksum: 4d0e47201afc7dde96c9fa15d2c21201 (MD5) S0102-695X2008000500002.pdf.txt: 31110 bytes, checksum: 1781a0ec1a98b12664c1dba22c8ea1d2 (MD5) Previous issue date: 2008-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Extratos brutos de 17 espécies de plantas coletadas em região de Mata Atlântica no Estado de São Paulo (Brasil) foram avaliadas para as atividades: antifúngica, no reparo do DNA e inibição da acetilcolinesterase. Dos 34 extratos obtidos de folhas e galhos das plantas analisadas para a atividade antifúngica com Cladosporium sphaerospermum e C. cladosporioides, 26,5% foram ativos. Todavia, apenas o extrato das folhas de Cabralea canjerana inibiu fortemente o crescimento dos dois fungos. No ensaio de reparo do DNA com linhagens mutantes de Saccharomyces cerevisiae, 11,7% dos extratos foram ativos, sendo que, 100% destes foram seletivos para o mecanismo de reparo do DNA envolvendo topoisomerase II. Das 17 espécies analisadas, 12 demonstraram atividade anticolinesterásica no ensaio qualitativo sobre cromatografia de camada delgada (CCD). No entanto, apenas os extratos de Tetrastylidium grandifolium (galhos) e Sloanea guianensis (folhas e galhos) apresentaram atividade anticolinesterásica maior que 50% no ensaio quantitativo. Crude extracts from 17 plant species collected from an Atlantic Forest region in the State of São Paulo (Brazil) have been screened for antifungal, DNA-damaging and acetylcholinesterase inhibiting activities. of the 34 extracts obtained from leaves and stems of plants assayed for antifungal activity with Cladosporium sphaerospermum and C. cladosporioides 26.5% were active. However, only the extract of leaves of Cabralea canjerana showed a strong inhibition of both fungi. The DNA-damaging assay with mutant strains of Saccharomyces cerevisiae resulted in 11.7 % of the extracts being active whereas 100% of them showed selectivity for the DNA-repair mechanism of topoisomerase II. of the 17 species analysed, 12 showed anticholinesterasic activity in TLC assay. However, only extracts from Tetrastylidium grandifolium (stems) and Sloanea guianensis (leaves and stems) inhibited acetylcholinesterase activity more than 50% in quantitative assay. Instituto de Botânica Universidade Estadual Paulista Instituto de Química Universidade Estadual Paulista Instituto de Química FAPESP: 03/02176-7 |
| Databáze: | OpenAIRE |
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