Comparative Gene Expression Profiling of Primary and Metastatic Renal Cell Carcinoma Stem Cell-Like Cancer Cells

Autor: Magdalena Król, Cezary Szczylik, Igor Helbrecht, Anna M. Czarnecka, Irena Koch, Klaudia Brodaczewska, Mohammed Imran Khan, Sławomir Lewicki, Robert Zdanowski
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Pathology
Cell Lines
Gene Expression
lcsh:Medicine
0302 clinical medicine
Cell Signaling
Animal Cells
Tumor Cells
Cultured

Medicine and Health Sciences
AC133 Antigen
lcsh:Science
Oligonucleotide Array Sequence Analysis
Staining
Multidisciplinary
biology
Stem Cells
Endoglin
Signaling cascades
Cell Staining
Nanog Homeobox Protein
Kidney Neoplasms
Oncology
030220 oncology & carcinogenesis
Neoplastic Stem Cells
CD146
Biological Cultures
Cellular Types
Anatomy
Stem cell
Network Analysis
Research Article
Signal Transduction
Homeobox protein NANOG
Computer and Information Sciences
medicine.medical_specialty
Epithelial-Mesenchymal Transition
Signal Inhibition
Research and Analysis Methods
Carcinomas
03 medical and health sciences
Cancer stem cell
Spheroids
Cellular

Biomarkers
Tumor

Genetics
medicine
Humans
CD90
Progenitor cell
Carcinoma
Renal Cell

Gene Expression Profiling
CD44
lcsh:R
Renal Cell Carcinoma
Cancers and Neoplasms
Biology and Life Sciences
Kidneys
Cell Biology
Renal System
Signaling Networks
Genitourinary Tract Tumors
030104 developmental biology
TGF-beta signaling cascade
Specimen Preparation and Treatment
Cancer cell
biology.protein
Cancer research
lcsh:Q
Octamer Transcription Factor-3
Stem Cell Lines
Zdroj: PLoS ONE, Vol 11, Iss 11, p e0165718 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: Background Recent advancement in cancer research has shown that tumors are highly heterogeneous, and multiple phenotypically different cell populations are found in a single tumor. Cancer development and tumor growth are driven by specific types of cells—stem cell-like cancer cells (SCLCCs)—which are also responsible for metastatic spread and drug resistance. This research was designed to verify the presence of SCLCCs in renal cell cancer cell lines. Subsequently, we aimed to characterize phenotype and cell biology of CD105+ cells, defined previously as renal cell carcinoma tumor-initiating cells. The main goal of the project was to describe the gene-expression profile of stem cell-like cancer cells of primary tumor and metastatic origin. Materials and Methods Real-time PCR analysis of stemness genes (Oct-4, Nanog and Ncam) and soft agar colony formation assay were conducted to check the stemness properties of renal cell carcinoma (RCC) cell lines. FACS analysis of CD105+ and CD133+ cells was performed on RCC cells. Isolated CD105+ cells were verified for expression of mesenchymal markers—CD24, CD146, CD90, CD73, CD44, CD11b, CD19, CD34, CD45, HLA-DR and alkaline phosphatase. Hanging drop assay was used to investigate CD105+ cell-cell cohesion. Analysis of free-floating 3D spheres formed by isolated CD105+ was verified, as spheres have been hypothesized to contain undifferentiated multipotent progenitor cells. Finally, CD105+ cells were sorted from primary (Caki-2) and metastatic (ACHN) renal cell cancer cell lines. Gene-expression profiling of sorted CD105+ cells was performed with Agilent’s human GE 4x44K v2 microarrays. Differentially expressed genes were further categorized into canonical pathways. Network analysis and downstream analysis were performed with Ingenuity Pathway Analysis. Results Metastatic RCC cell lines (ACHN and Caki-1) demonstrated higher colony-forming ability in comparison to primary RCC cell lines. Metastatic RCC cell lines harbor numerous CD105+ cell subpopulations and have higher expression of stemness genes (Oct-4 and Nanog). CD105+ cells adopt 3D grape-like floating structures under handing drop conditions. Sorted CD105+ cells are positive for human mesenchymal stem cell (MSC) markers CD90, CD73, CD44, CD146, and alkaline phosphatase activity, but not for CD24 and hematopoietic lineage markers CD34, CD11b, CD19, CD45, and HLA-DR. 1411 genes are commonly differentially expressed in CD105+ cells (both from primary [Caki-2] and metastatic RCC [ACHN] cells) in comparison to a healthy kidney epithelial cell line (ASE-5063). TGF-β, Wnt/β-catenine, epithelial-mesenchymal transition (EMT), Rap1 signaling, PI3K-Akt signaling, and Hippo signaling pathway are deregulated in CD105+ cells. TGFB1, ERBB2, and TNF are the most significant transcriptional regulators activated in these cells. Conclusions All together, RCC-CD105+ cells present stemlike properties. These stem cell-like cancer cells may represent a novel target for therapy. A unique gene-expression profile of CD105+ cells could be used as initial data for subsequent functional studies and drug design.
Databáze: OpenAIRE