Induction of human mammary-associated serum amyloid A3 expression by prolactin or lipopolysaccharide
| Autor: | Annika Weber, Shu H. Wei, Allen T. Weber, Marilynn A. Larson, Thomas L. McDonald |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Lipopolysaccharides
Gene isoform DNA Complementary Molecular Sequence Data Biophysics Gene Expression Biology Biochemistry Cell Line Rapid amplification of cDNA ends Downregulation and upregulation Sequence Homology Nucleic Acid Complementary DNA Gene expression Humans Protein Isoforms Breast Serum amyloid A Molecular Biology Gene DNA Primers Serum Amyloid A Protein Base Sequence Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Epithelial Cells Cell Biology Molecular biology Prolactin Female |
| Zdroj: | Biochemical and Biophysical Research Communications. 301:1030-1037 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(03)00045-7 |
| Popis: | In most mammalian species, serum amyloid A isoform 3 (SAA3) appears to be the predominant SAA isoform expressed extrahepatically. However, human SAA3 gene expression has not been detected previously and, therefore, this gene was referred to as a pseudogene. We report for the first time the transcriptional expression of human SAA3. Human SAA3 gene expression was detected by RT-PCR after stimulation of mammary gland epithelial cells with either prolactin (PRL) or lipopolysaccharide (LPS). The full-length 655bp cDNA sequence for this mammary-associated serum amyloid A3 (M-SAA3) was obtained using 5' and 3' rapid amplification of cDNA ends (RACE). The human M-SAA3 transcript would conceptually translate into a 42 residue mature protein, which is smaller than other mammalian SAA3 isoforms that are typically 104-113 amino acids in length. This study defines the cDNA sequence for human SAA3 and also demonstrates the upregulation of M-SAA3 expression in response to the lactational hormone PRL or to an acute phase stimulant such as LPS. |
| Databáze: | OpenAIRE |
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