| Autor: |
Ulrich Hagen, Gisela Schäfer, Annemarie Schön-Bopp |
| Rok vydání: |
1977 |
| Předmět: |
|
| Zdroj: |
International journal of radiation biology and related studies in physics, chemistry, and medicine. 31(3) |
| ISSN: |
0020-7616 |
| Popis: |
Several protein fractions containing endonuclease activity against gemma-irradiated DNA (gamma-endonuclease) were isolated from M. luteus. The crude extract was eluted on a phosphocellulose column and chromatographed on TEAE cellulose and subsequently on hydroxyapatite. Five peaks of gamma-endonuclease were obtained from each preparation. Repeated experiments showed comparable chromatographic behavior of the fractions. There was no detectable activity of U.V.-endonuclease in the fractions with gamma-endonuclease but a small contamination of endonuclease against unirradiated DNA and against DNA with apurinic sites. The gamma-endonuclease is stimulated by, but is not dependent on, magnesium. Several tests for endonuclease activity have been used: the analysis of strand breaks in calf-thymus DNA or in PM2 DNA, and the determination of end-groups formed by endonuclease, either 3'OH end-groups or phosphomonoester end groups. From the results obtained it can be assumed that the strand breaks induced by the gamma-endonuclease carry 3'OH and 5' phosphate end groups. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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