PSGL-1-Mediated Adhesion of Human Hematopoietic Progenitors to P-Selectin Results in Suppression of Hematopoiesis
| Autor: | Michael C. Berndt, Paul J. Simmons, Melanie Pudney, Geoffrey S. Kansas, Karen R. Snapp, Silvana Niutta, Jean-Pierre Levesque, David N. Haylock, Andrew C.W. Zannettino |
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| Jazyk: | angličtina |
| Předmět: |
P-selectin
Immunology CD34 Stem cell factor Antigens CD34 Apoptosis Bone Marrow Cells CHO Cells Biology Ligands 03 medical and health sciences 0302 clinical medicine NAD+ Nucleosidase Antigens CD Cricetinae Granulocyte Colony-Stimulating Factor Cell Adhesion Immunology and Allergy Animals Humans Progenitor cell Receptor Cell adhesion ADP-ribosyl Cyclase Cells Cultured 030304 developmental biology Interleukin 3 0303 health sciences Stem Cell Factor Membrane Glycoproteins integumentary system Interleukin-6 Hematopoietic Stem Cells ADP-ribosyl Cyclase 1 Antigens Differentiation Cell biology Hematopoiesis Haematopoiesis P-Selectin Infectious Diseases Solubility Interleukin-3 Cell Division 030215 immunology |
| Zdroj: | Immunity. (3):369-378 |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/S1074-7613(00)80112-0 |
| Popis: | Cellular interactions are critical for the regulation of hematopoiesis. The sialomucin PSGL-1/CD162 mediates the attachment of mature leukocytes to P-selectin. We now show that PSGL-1 also functions as the sole receptor for P-selectin on primitive human CD34+ hematopoietic progenitor cells (HPC). More importantly, ligation of PSGL-1 by immobilized or soluble ligand or anti-PSGL-1 antibody results in a profound suppression of HPC proliferation stimulated by potent combinations of early acting hematopoietic growth factors. These data demonstrate an unanticipated but extremely marked growth-inhibitory effect of P-selectin on hematopoiesis and provide direct evidence that PSGL-1, in addition to its well-documented role as an adhesion molecule on mature leukocytes, is a potent negative regulator of human hematopoietic progenitors. |
| Databáze: | OpenAIRE |
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