Nucleotide sequence of the Aspergillus niger trpC gene: structural relationship with analogous genes of other organisms
| Autor: | Pouwels Ph, Kuijvenhoven A, van den Hondel Ca, Kos T, Hessing Hg |
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| Rok vydání: | 1988 |
| Předmět: |
Base Sequence
Sequence analysis Genes Fungal Molecular Sequence Data Nucleic acid sequence Intron Tryptophan General Medicine DNA Restriction Enzymes Biology Molecular biology Conserved sequence Biochemistry Genes Species Specificity Sequence Homology Nucleic Acid Genes Regulator Genetics Amino Acid Sequence Aspergillus niger Promoter Regions Genetic Gene Peptide sequence TRPC Glutamine amidotransferase |
| Zdroj: | Current genetics. 13(2) |
| ISSN: | 0172-8083 |
| Popis: | The nucleotide sequence of the Aspergillus niger tryptophan C (trpC) gene was determined. Northern hybridization and S1-mapping experiments showed the presence of a 2.6 kb trpC poly(A)+ RNA with two very short (5 and 6 nucleotides) noncoding 5′-regions. Comparison of the predicted amino acid sequence with that of trp gene proteins of pro- and eukaryotic organisms revealed three functional domains (G, C, F) in the A. niger TrpC protein which catalyse the glutarnine amidotransferase reaction (GAT), the indole-3-glycerol phosphate synthase reaction (IGPS) and the N-(5′-phosphoribosyl) anthranilate isomerase reaction (PRAI), respectively. These domains are highly conserved and bordered by short areas showing less homology. Within the F domain of the trpC gene in A. niger, A. nidulans and Neurospora crassa, a region encoding 30 amino acids was found which is absent in the analogous genes of Saccharomyces cerevisiae and prokaryotic organisms. This region has features of a mutated in-phase intron. |
| Databáze: | OpenAIRE |
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