The Prognostic Value of the Detection of Microbial Translocation in the Blood of Colorectal Cancer Patients

Autor:	John Souglakos, Dimitrios Mavroudis, Asimina Koulouridi, Konstantinos Vogiatzoglou, Ippokratis Messaritakis, Konstantina Tsantaki, Agapi Ntretaki, Maria Sfakianaki, John Tsiaoussis
Rok vydání:	2020
Předmět:	0301 basic medicine Cancer Research microbial translocation Microbial DNA Colorectal cancer Chromosomal translocation colorectal cancer lcsh:RC254-282 Calcitriol receptor Article 03 medical and health sciences 0302 clinical medicine medicine bacterial translocation Gene biology vitamin D receptors dysbiosis lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens biology.organism_classification medicine.disease Molecular biology genomic DNA 030104 developmental biology Oncology toll-like receptors 030220 oncology & carcinogenesis Bacteroides fragilis Dysbiosis
Zdroj:	Cancers Volume 12 Issue 4 Cancers, Vol 12, Iss 1058, p 1058 (2020)
ISSN:	2072-6694
Popis:	Dysbiosis has been associated with various diseases and is of major health importance. Dysbiosis leads to microbial translocation, which is the passage of microorganisms, their fragments, or their metabolites from the intestinal lumen into the blood circulation and other sites. The aim of the study was to determine whether microbial translocation occurs in stage II/III-IV colorectal cancer (CRC) patients. The aim was also to evaluate the usefulness of blood PCR for diagnosis of such translocation and correlate the presence of toll-like receptor/vitamin D receptor (TLR/VDR) gene polymorphisms with microbial DNA fragments detected in the blood of CRC patients. Three hundred and ninety-seven CRC patients enrolled in the study. Peripheral blood DNA was analyzed using PCR for the amplification of genomic DNA encoding 16S rRNA, the &beta galactosidase gene of Escherichia coli, glutamine synthase gene of Bacteroides fragilis, and 5.8S rRNA of Candida albicans. Significantly higher rates of all microbial fragments, but E. coli, detected were observed in the CRC patients (p < 0.001) such detection of all four microbial fragments was also significantly associated with the metastatic disease (p < 0.001), leading to shorter survival rates (p < 0.001). Tumor location in the right colon also significantly correlated with shorter survival (p = 0.016). Individuals with homozygous mutant alleles of TLR/VDR polymorphisms had significantly higher detection rates of microbial DNA fragments. The detection of microbial DNA fragments in CRC patients highlighted the role of these microbes in cancer development, progression, and patients&rsquo survival.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77d04db76a5fc8c9e52eea9dc2f092bf https://pubmed.ncbi.nlm.nih.gov/32344707 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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