Tobacco mosaic virus in the lungs of mice following intra-tracheal inoculation

Autor: Audrey Ferretti, Hervé Lecoq, Abdoulaye Oury Barry, Didier Raoult, Fanny Balique, Tatsiana Ngounga, Claude Nappez, Khatoun Al Moussawi, Jean-Louis Mege, Philippe Colson, Eric Ghigo, Hubert Lepidi
Přispěvatelé: Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), Université de la Méditerranée - Aix-Marseille 2, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Assistance Publique - Hôpitaux de Marseille (APHM), the Centre National de la Recherche Scientifique (CNRS), Colson, Philippe
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Mouse
santé du consommateur
viruses
lcsh:Medicine
Pathogenesis
Plant Science
souris
Antibodies
Viral
Mice
Plant Microbiology
Tobacco mosaic virus
lcsh:Science
interaction virus homme
Lung
Infectivity
0303 health sciences
Multidisciplinary
medicine.diagnostic_test
biology
food and beverages
persistence
Animal Models
3. Good health
Tobacco Mosaic Virus
Trachea
Santé publique et épidémiologie
anticorps
Antibody
Bronchoalveolar Lavage Fluid
Research Article
virus de la mosaïque du tabac
Plant Pathogens
Bone Marrow Cells
Immunofluorescence
Microbiology
Virus
03 medical and health sciences
Model Organisms
Plant virus
Virology
medicine
rt pcr
Animals
Serologic Tests
immunofluorescence
Biology
pénétration virale
030304 developmental biology
virus phytopathogène
Microbial Viability
030306 microbiology
Inoculation
Macrophages
lcsh:R
fungi
Plant Pathology
Bronchoalveolar lavage
Emerging Infectious Diseases
poumon
biology.protein
lcsh:Q
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
détection de virus
Zdroj: PLoS ONE
PLoS ONE, 2013, 8 (1), pp.e54993. ⟨10.1371/journal.pone.0054993⟩
PLoS ONE, Public Library of Science, 2013, 8 (1), pp.e54993. ⟨10.1371/journal.pone.0054993⟩
Plos One 1 (8), e54993. (2013)
PLoS ONE, Vol 8, Iss 1, p e54993 (2013)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0054993⟩
Popis: International audience; Plant viruses are generally considered incapable of infecting vertebrates. Accordingly, they are not considered harmful for humans. However, a few studies questioned the certainty of this paradigm. Tobacco mosaic virus (TMV) RNA has been detected in human samples and TMV RNA translation has been described in animal cells. We sought to determine if TMV is detectable, persists, and remains viable in the lung tissues of mice following intratracheal inoculation, and we attempted to inoculate mouse macrophages with TMV. In the animal model, mice were intratracheally inoculated with 10(11) viral particles and were sacrificed at different time points. The virus was detected in the mouse lungs using immunohistochemistry, electron microscopy, real-time RT-PCR and sequencing, and its viability was studied with an infectivity assay on plants. In the cellular model, the culture medium of murine bone marrow derived macrophages (BMDM) was inoculated with different concentrations of TMV, and the virus was detected with real-time RT-PCR and immunofluorescence. In addition, anti-TMV antibodies were detected in mouse sera with ELISA. We showed that infectious TMV could enter and persist in mouse lungs via the intratracheal route. Over 14 days, the TMV RNA level decreased by 5 log(10) copies/ml in the mouse lungs and by 3.5 log(10) in macrophages recovered from bronchoalveolar lavage. TMV was localized to lung tissue, and its infectivity was observed on plants until 3 days after inoculation. In addition, anti-TMV antibody seroconversions were observed in the sera from mice 7 days after inoculation. In the cellular model, we observed that TMV persisted over 15 days after inoculation and it was visualized in the cytoplasm of the BMDM. This work shows that a plant virus, Tobacco mosaic virus, could persist and enter in cells in mammals, which raises questions about the potential interactions between TMV and human hosts.
Databáze: OpenAIRE
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