Is maintenance of the ileocecal valve important to the intestinal adaptation mechanisms in a weaning rat model of short bowel?

Autor: Uenis Tannuri, Vitor Van Vaisberg, Ana Cristina Aoun Tannuri, Ítalo Gerardo Rotondo, Cícero Mendes Neto, Leandro Silveira Sarmento, Maria Cecília Mendonça Coelho, Josiane de Oliveira Gonçalves, Suellen Serafini, Guilherme Garcia Barros
Rok vydání: 2018
Předmět:
Short Bowel Syndrome
medicine.medical_specialty
Colon
medicine.medical_treatment
Crypt
bcl-X Protein
Weight Gain
digestive system
Gastroenterology
Rats
Sprague-Dawley
03 medical and health sciences
Ileocecal valve
0302 clinical medicine
030225 pediatrics
Internal medicine
Intestine
Small
medicine
Weaning
Animals
RNA
Messenger
Lamina propria
Ileocecal Valve
Mucous Membrane
business.industry
digestive
oral
and skin physiology
Intestinal villus
General Medicine
Bowel resection
Short bowel syndrome
medicine.disease
Adaptation
Physiological
digestive system diseases
Disease Models
Animal
medicine.anatomical_structure
Ki-67 Antigen
Pediatrics
Perinatology and Child Health
INTESTINO DE ANIMAL
030211 gastroenterology & hepatology
Surgery
medicine.symptom
business
Weight gain
Organ Sparing Treatments
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1437-9813
Popis: To evaluate the role of maintenance of the ileocecal valve (ICV) in intestinal adaptation mechanisms, in a weaning rat experimental model of short bowel. Forty animals were operated on to produce short bowel syndrome. They were divided into five groups: maintenance (MV) or resection of ICV (RV), kill after 4 days (MV4 and RV4) or 21 days (MV21 and RV21), and a control group (21-day-old rats). Body weights, small bowel and colon lengths and diameters, villus heights, crypt depths, lamina propria and muscle layer thickness, as well as the apoptosis index of villi and crypts and expression of pro- and anti-apoptotic genes, were studied. Preservation of the ICV promoted increased weight gain (p = 0.0001) and intestinal villus height after 21 days; crypt depth was higher in comparison to controls. It was verified a higher expression of Ki-67 in bowel villi and crypts (p = 0.018 and p = 0.015, respectively) in RV4 group and a higher expression in bowel villi of MV4 group animals (p = 0.03). The maintenance of ICV promoted late increased expression of the anti-apoptotic gene Bcl-XL in the colon (p = 0.043, p = 0.002, p = 0.01). The maintenance of the ICV led to positive changes in this model.
Databáze: OpenAIRE
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