N-Ethylmaleimide-sensitive Factor (NSF) and α-Soluble NSF Attachment Proteins (SNAP) Mediate Dissociation of GS28-Syntaxin 5 Golgi SNAP Receptors (SNARE) Complex
| Autor: | V. N. Subramaniam, Eva Loh, Wanjin Hong |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
endocrine system
Macromolecular Substances Vesicular Transport Proteins Golgi Apparatus Nerve Tissue Proteins environment and public health Biochemistry symbols.namesake ATP hydrolysis Animals Syntaxin Fluorescent Antibody Technique Indirect Receptor N-Ethylmaleimide-Sensitive Proteins Molecular Biology Integral membrane protein Qa-SNARE Proteins urogenital system Chemistry Membrane Proteins Cell Biology Golgi apparatus Cell biology Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Vesicular transport protein Microscopy Fluorescence nervous system symbols Electrophoresis Polyacrylamide Gel Rabbits biological phenomena cell phenomena and immunity Carrier Proteins SNARE Proteins SNARE complex |
| Zdroj: | Journal of Biological Chemistry. 272:25441-25444 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.272.41.25441 |
| Popis: | Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) GS28 and syntaxin 5 can be reciprocally coimmunoprecipitated from Golgi extracts, suggesting that they exist in a protein complex. When Golgi extract is preincubated with soluble NSF attachment proteins (alpha-SNAP) and N-ethylmaleimide-sensitive factor (NSF) under conditions that allow ATP hydrolysis by NSF, GS28 and syntaxin 5 become dissociated. GS28 and syntaxin 5 remain in a protein complex when Golgi extract is preincubated with similar amounts of alpha-SNAP and NSF under conditions that prevent ATP hydrolysis by NSF, suggesting that ATP hydrolysis by NSF is necessary for dissociating the GS28-syntaxin 5 complex. Since preincubation of Golgi extract with either alpha-SNAP or NSF alone has no effect on the GS28-syntaxin 5 complex, a concerted action of alpha-SNAP and NSF therefore mediates the dissociation of the GS28-syntaxin 5 complex. Furthermore, GS28 but not syntaxin 5 is capable of binding to immobilized alpha-SNAP when the GS28-syntaxin 5 complex is dissociated. |
| Databáze: | OpenAIRE |
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