An antigen microarray protocol for COVID-19 serological analysis
| Autor: | Gunnar Dittmar, Joseph Longworth |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Science (General) Microarray Coronavirus disease 2019 (COVID-19) Bioinformatics High-throughput screening Immunology Antibodies Viral General Biochemistry Genetics and Molecular Biology Serology Q1-390 Antigen Health Sciences Protocol Humans Antigens Viral Antibody General Immunology and Microbiology biology SARS-CoV-2 General Neuroscience COVID-19 Microarray Analysis Virology High Throughput Screening Spike Glycoprotein Coronavirus biology.protein DNA microarray |
| Zdroj: | STAR Protocols, Vol 2, Iss 3, Pp 100815-(2021) STAR Protocols |
| ISSN: | 2666-1667 |
| Popis: | The emergence of the Covid-19 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used, but are restricted by a single response value to an antigen or antigen pool. Here we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest. Graphical Abstract |
| Databáze: | OpenAIRE |
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