Phospholipase A and glycerophosphodiesterase activities in the cell membrane of Mycoplasma hyorhinis
| Autor: | Jonathan D. Kornspan, Shlomo Rottem |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Mycoplasma hyorhinis
Molecular Sequence Data Phospholipase Biology medicine.disease_cause Microbiology Phospholipases A Cell membrane Tissue culture Bacterial Proteins Genetics medicine Amino Acid Sequence Molecular Biology Fluorescent Dyes chemistry.chemical_classification Host cell membrane Phospholipase A Phosphoric Diester Hydrolases Hydrolysis Cell Membrane Mycoplasma biology.organism_classification 4-Chloro-7-nitrobenzofurazan medicine.anatomical_structure Enzyme chemistry Biochemistry Phosphatidylcholines Sequence Alignment |
| Zdroj: | FEMS Microbiology Letters. 332:34-39 |
| ISSN: | 0378-1097 |
| Popis: | Mycoplasma hyorhinis, the major contaminant of tissue cultures, has been implicated in a variety of diseases in swine. Most human and animal mycoplasmas remain attached to the surface of epithelial cells. Nonetheless, we have recently shown that M. hyorhinis is able to invade and survive within nonphagocytic melanoma cells. The invasion process may require the damaging of the host cell membrane by either chemical, physical or enzymatic means. In this study, we show that M. hyorhinis membranes possess a nonspecific phospholipase A (PLA) activity capable of hydrolyzing both position 1 and position 2 of 1-acyl-2-(12-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)] aminododecanoyl) phosphatidylcholine. In silico analysis of the M. hyorhinis genome shows that the PLA of M. hyorhinis shares no homology to described phospholipases. The PLA activity of M. hyorhinis was neither stimulated by Ca (2+) nor inhibited by EGTA and had a broad pH spectrum. Mycoplasma hyorhinis also possess a potent glycerophosphodiesterase (GPD), which apparently cleaves the glycerophosphodiester formed by PLA to yield glycerol-3-phosphate. Possible roles of PLA and GPD in invading host eukaryotic cells and in forming mediators upon the interaction of M. hyorhinis with eukaryotic cells are suggested. |
| Databáze: | OpenAIRE |
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