Dissimilatory Reduction of Fe(III) and Other Electron Acceptors by a Thermus Isolate
| Autor: | T. J. Bailey, Tullis C. Onstott, Andrew E. Plymale, James K. Fredrickson, Thomas L. Kieft, M. S. Gray, David W. Kennedy, Yuri A. Gorby, S. W. Li, Heather M. Kostandarithes, Christina M. Spadoni |
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| Rok vydání: | 1999 |
| Předmět: |
DNA
Bacterial Nitrilotriacetic Acid Molecular Sequence Data chemistry.chemical_element Fresh Water Manganese engineering.material DNA Ribosomal Ferric Compounds Applied Microbiology and Biotechnology Hydrous ferric oxides chemistry.chemical_compound RNA Ribosomal 16S Humic acid Thermus Phylogeny chemistry.chemical_classification Ecology biology Strain (chemistry) Thermophile Temperature Nitrilotriacetic acid Genes rRNA General Microbial Ecology Electron acceptor biology.organism_classification Biodegradation Environmental chemistry Biochemistry Lactates engineering Water Microbiology Oxidation-Reduction Food Science Biotechnology Nuclear chemistry |
| Zdroj: | Applied and Environmental Microbiology. 65:1214-1221 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.65.3.1214-1221.1999 |
| Popis: | A thermophilic bacterium that can use O 2 , NO 3 − , Fe(III), and S 0 as terminal electron acceptors for growth was isolated from groundwater sampled at a 3.2-km depth in a South African gold mine. This organism, designated SA-01, clustered most closely with members of the genus Thermus , as determined by 16S rRNA gene (rDNA) sequence analysis. The 16S rDNA sequence of SA-01 was >98% similar to that of Thermus strain NMX2 A.1, which was previously isolated by other investigators from a thermal spring in New Mexico. Strain NMX2 A.1 was also able to reduce Fe(III) and other electron acceptors. Neither SA-01 nor NMX2 A.1 grew fermentatively, i.e., addition of an external electron acceptor was required for anaerobic growth. Thermus strain SA-01 reduced soluble Fe(III) complexed with citrate or nitrilotriacetic acid (NTA); however, it could reduce only relatively small quantities (0.5 mM) of hydrous ferric oxide except when the humic acid analog 2,6-anthraquinone disulfonate was added as an electron shuttle, in which case 10 mM Fe(III) was reduced. Fe(III)-NTA was reduced quantitatively to Fe(II); reduction of Fe(III)-NTA was coupled to the oxidation of lactate and supported growth through three consecutive transfers. Suspensions of Thermus strain SA-01 cells also reduced Mn(IV), Co(III)-EDTA, Cr(VI), and U(VI). Mn(IV)-oxide was reduced in the presence of either lactate or H 2 . Both strains were also able to mineralize NTA to CO 2 and to couple its oxidation to Fe(III) reduction and growth. The optimum temperature for growth and Fe(III) reduction by Thermus strains SA-01 and NMX2 A.1 is approximately 65°C; their optimum pH is 6.5 to 7.0. This is the first report of a Thermus sp. being able to couple the oxidation of organic compounds to the reduction of Fe, Mn, or S. |
| Databáze: | OpenAIRE |
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