Polyphenolic constituents, antioxidant and hypoglycaemic potential of leaf extracts of Acalypha godseffiana from Eastern Nigeria: In vitro study
| Autor: | Omobola O. Okoh, Anofi Omotayo Tom Ashafa, Oluwole B. Familoni, Olayinka T. Asekun, Anita K. Asekunowo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Economics and Econometrics
antioxidant Flavonoid lcsh:RX1-681 Plant Science 01 natural sciences chemistry.chemical_compound lcsh:Homeopathy Phenols Gallic acid chemistry.chemical_classification Acalypha Acalypha godeseffiana biology Traditional medicine 010405 organic chemistry Chemistry Catechin biology.organism_classification phytochemicals 0104 chemical sciences hypoglycemic 010404 medicinal & biomolecular chemistry Phytochemical Polyphenol acetone extract Quercetin |
| Zdroj: | Journal of Medicinal Plants for Economic Development, Vol 3, Iss 1, Pp e1-e9 (2019) |
| ISSN: | 2616-4809 |
| Popis: | Background: Acalypha godseffiana is an important plant used as an ornamental and herbs; its leaves are used in the management of diseases like diabetes in Eastern Nigeria. Aim: The study aims at linking the hypoglycemic activity extracts of leaves of A. godseffiana to their polyphenolic contents. Setting: Fresh leaves of A. godseffiana were obtainedfrom Imo State, Nigeria, identified in University of Lagos Herbarium, air-dried, pulverized and kept for investigations. Method: The phytochemical compositions and antioxidant potentials of acetone, aqueous, ethanol and methanol extracts of A. godseffiana were determined using adopted methods. An in-vitro approach was used to evaluate the hypoglycemic potentials of the extracts on α-amylase and α-glucosidase enzymes. The mechanism of inhibitions was studied using the Lineweaver-Burk plot. Results: Antioxidant results revealed that total antioxidant capacity of the acetone extract (IC50: 0.34 mg/mL) showed better activity compared to the standards (silymarin 0.52 mg/mL; gallic acid 0.51 mg/mL). Thehypoglycemic findings confirmed that acetone extract demonstrated strong and mild inhibitory potential against α-amylase and α-glucosidase respectively, showing concentration dependent with IC 50 values of 2.33 mg/mL and 0.13 mg/mL. The observed hypoglycemic and antioxidant potentials of acetone extract of A. godseffiana correlate to its high polyphenolic contents which include phenols (133.20 mg gallic acid g -1 ), flavonoid (350.60 mg quercetin g -1 ) and tannins (264.67 mg catechin g -1 ). The mechanisms of action exhibited by the acetone extractwere uncompetitive and mixed non-competitive which can be attributed to its inhibitory properties on α-glucosidase and α-amylase respectively. Conclusion: The results obtained from this study validate the acetone leaves extract of A. godseffiana as potential agent in management of sugar related disorder. |
| Databáze: | OpenAIRE |
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