Complete genomic sequence of the virulent Salmonella bacteriophage SP6
| Autor: | Daryl A. Basham, Marisa L. Pedulla, Jeffrey G. Lawrence, Graham F. Hatfull, Michael E. Ford, Aleisha T. Dobbins, Matthew George, Roger W. Hendrix, Jennifer M. Houtz |
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| Rok vydání: | 2004 |
| Předmět: |
DNA Replication
Gene Expression Regulation Viral Salmonella typhimurium Sequence analysis viruses Molecular Sequence Data Bacteriophages Transposons and Plasmids Genomics Genome Viral Biology Microbiology Genome chemistry.chemical_compound Open Reading Frames Viral Proteins RNA polymerase Coding region Amino Acid Sequence Promoter Regions Genetic Molecular Biology Gene Peptide sequence Phylogeny Genetics Terminator Regions Genetic Base Sequence fungi Sequence Analysis DNA Virology chemistry Salmonella Phages DNA |
| Zdroj: | Journal of bacteriology. 186(7) |
| ISSN: | 0021-9193 |
| Popis: | We report the complete genome sequence of enterobacteriophage SP6, which infects Salmonella enterica serovar Typhimurium. The genome contains 43,769 bp, including a 174-bp direct terminal repeat. The gene content and organization clearly place SP6 in the coliphage T7 group of phages, but there is ∼5 kb at the right end of the genome that is not present in other members of the group, and the homologues of T7 genes 1.3 through 3 appear to have undergone an unusual reorganization. Sequence analysis identified 10 putative promoters for the SP6-encoded RNA polymerase and seven putative rho-independent terminators. The terminator following the gene encoding the major capsid subunit has a termination efficiency of about 50% with the SP6-encoded RNA polymerase. Phylogenetic analysis of phages related to SP6 provided clear evidence for horizontal exchange of sequences in the ancestry of these phages and clearly demarcated exchange boundaries; one of the recombination joints lies within the coding region for a phage exonuclease. Bioinformatic analysis of the SP6 sequence strongly suggested that DNA replication occurs in large part through a bidirectional mechanism, possibly with circular intermediates. |
| Databáze: | OpenAIRE |
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