Laboratory diagnosis of nontuberculous mycobacteria in a Belgium Hospital

Autor: Alexandre Colmant, Alexia Verroken, Hector Rodriguez-Villalobos, Anandi Martin
Přispěvatelé: UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - (SLuc) Service de microbiologie
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Microbiology (medical)
medicine.medical_specialty
diagnosis
mycobacteria
Culture
lcsh:QR1-502
Mycobacterium chelonae
Mycobacterium Infections
Nontuberculous
Mycobacterium gordonae
lcsh:Microbiology
03 medical and health sciences
0302 clinical medicine
Belgium
Internal medicine
Medicine
Humans
Lung
Retrospective Studies
biology
business.industry
Clinical Laboratory Techniques
matrix-assisted laser desorption ionization-time of flight
Mycobacterium abscessus complex
Routine work
Sputum
High-Throughput Nucleotide Sequencing
Nontuberculous Mycobacteria
sequencing
biology.organism_classification
Laboratories
Hospital
Mycobacterium avium Complex
bacterial infections and mycoses
Clinical Practice
Infectious Diseases
030220 oncology & carcinogenesis
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Proper treatment
Nontuberculous mycobacteria
business
030215 immunology
Mycobacterium
Zdroj: International Journal of Mycobacteriology, Vol 8, Iss 2, Pp 157-161 (2019)
International journal of mycobacteriology, Vol. 8, no.2, p. 157-161 (2019)
ISSN: 2212-5531
Popis: Background: Nontuberculous mycobacteria (NTM) have been identified in human pulmonary and extrapulmonary infections and are increasing globally, which makes it challenging to identify them. This article reports our experience with the laboratory identification of NTM in clinical practice among pulmonary and extrapulmonary samples received in our routine work. Methods: The study was conducted at the Universite Catholique de Louvain at the Cliniques Universitaires Saint-Luc, Brussels, Belgium, from 2015 to 2018. A total of 386 clinical samples were collected from patients suspected of having pulmonary or extrapulmonary mycobacterial infections. Routine laboratory methods phenotypic and molecular tests were performed. Results: The majority of NTM species were isolated from pulmonary samples (68%). The most prevalent species identified were Mycobacterium chimaera_intracellulare group (32%), followed by Mycobacterium avium complex (21%), Mycobacterium abscessus complex (18%), Mycobacterium gordonae (9%), and Mycobacterium chelonae (4%). In extrapulmonary samples, M. avium and M. chimaera_intracellulare were the most frequently isolated. Conclusion: The species diversity of NTM found in our setting suggests the importance of the use of new modern methods for accurate identification of NTM at species level and in some case at subspecies level for the proper treatment and management of patients.
Databáze: OpenAIRE
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