Escherichia coli Nissle 1917 administered as a dextranomar microsphere biofilm enhances immune responses against human rotavirus in a neonatal malnourished pig model colonized with human infant fecal microbiota
Autor: | Linda J. Saif, Stephanie N. Langel, Ayako Miyazaki, Juliet Chepngeno, Anastasia N. Vlasova, Francine C. Paim, Husheem Michael, Steven D. Goodman, Gireesh Rajashekara, David D. Fischer |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Rotavirus
B Cells Swine medicine.disease_cause Antibodies Viral law.invention Probiotic White Blood Cells Feces law Pig Models Animal Cells Medicine and Health Sciences Medicine Mammals B-Lymphocytes Multidisciplinary biology Microbiota Eukaryota Dextrans SOX9 Transcription Factor Animal Models Microspheres Up-Regulation Diarrhea medicine.anatomical_structure Experimental Organism Systems Vertebrates Antibody medicine.symptom Anatomy Cellular Types Research Article Science Immune Cells Immunology Gastroenterology and Hepatology Research and Analysis Methods Peripheral blood mononuclear cell Microbiology Immune system Signs and Symptoms Escherichia coli Animals RNA Messenger Antibody-Producing Cells B cell Blood Cells business.industry Probiotics Malnutrition Biofilm Organisms Biology and Life Sciences Bacteriology Cell Biology Gastrointestinal Tract Disease Models Animal Animals Newborn Biofilms Amniotes biology.protein Animal Studies Clinical Medicine business Bacterial Biofilms Zoology Digestive System |
Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 2, p e0246193 (2021) |
ISSN: | 1932-6203 |
Popis: | Human rotavirus (HRV) is a leading cause of diarrhea in children. It causes significant morbidity and mortality, especially in low- and middle-income countries (LMICs), where HRV vaccine efficacy is low. The probiotic Escherichia coli Nissle (EcN) 1917 has been widely used in the treatment of enteric diseases in humans. However, repeated doses of EcN are required to achieve maximum beneficial effects. Administration of EcN on a microsphere biofilm could increase probiotic stability and persistence, thus maximizing health benefits without repeated administrations. Our aim was to investigate immune enhancement by the probiotic EcN adhered to a dextranomar microsphere biofilm (EcN biofilm) in a neonatal, malnourished piglet model transplanted with human infant fecal microbiota (HIFM) and infected with rotavirus. To create malnourishment, pigs were fed a reduced amount of bovine milk. Decreased HRV fecal shedding and protection from diarrhea were evident in the EcN biofilm treated piglets compared with EcN suspension and control groups. Moreover, EcN biofilm treatment enhanced natural killer cell activity in blood mononuclear cells (MNCs). Increased frequencies of activated plasmacytoid dendritic cells (pDC) in systemic and intestinal tissues and activated conventional dendritic cells (cDC) in blood and duodenum were also observed in EcN biofilm as compared with EcN suspension treated pigs. Furthermore, EcN biofilm treated pigs had increased frequencies of systemic activated and resting/memory antibody forming B cells and IgA+ B cells in the systemic tissues. Similarly, the mean numbers of systemic and intestinal HRV-specific IgA antibody secreting cells (ASCs), as well as HRV-specific IgA antibody titers in serum and small intestinal contents, were increased in the EcN biofilm treated group. In summary EcN biofilm enhanced innate and B cell immune responses after HRV infection and ameliorated diarrhea following HRV challenge in a malnourished, HIFM pig model. |
Databáze: | OpenAIRE |
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