Isolation of filamentous fungi from sputum in asthma is associated with reduced post-bronchodilator FEV1
| Autor: | Neeta Kulkarni, J. P. Morley, Andrew J. Wardlaw, Joshua Agbetile, Catherine H. Pashley, Beverley Hargadon, R.E. Edwards, Michelle Bourne, Abbie Fairs, Dhananjay Desai, K Mutalithas, Christopher E. Brightling, Ian D. Pavord, Ruth H. Green, Peter Bradding, Will Monteiro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Spirometry Immunology airflow obstruction Immunoglobulin E medicine.disease_cause Anti-asthmatic Agent Microbiology Aspergillus fumigatus Young Adult fungal culture 03 medical and health sciences 0302 clinical medicine Allergen Phagocytosis Adrenal Cortex Hormones Forced Expiratory Volume medicine Humans Immunology and Allergy Anti-Asthmatic Agents Aged Asthma Aged 80 and over 0303 health sciences biology medicine.diagnostic_test 030306 microbiology Macrophages Fungi Sputum Original Articles Middle Aged asthma biology.organism_classification medicine.disease Bronchodilator Agents 3. Good health respiratory tract diseases fungal allergy 030228 respiratory system biology.protein Female medicine.symptom Allergic bronchopulmonary aspergillosis |
| Zdroj: | Clinical and Experimental Allergy |
| ISSN: | 1365-2222 0954-7894 |
| Popis: | SummaryBackground Fungal sensitization is common in severe asthma, but the clinical relevance of this and the relationship with airway colonization by fungi remain unclear. The range of fungi that may colonize the airways in asthma is unknown. Objective To provide a comprehensive analysis on the range of filamentous fungi isolated in sputum from people with asthma and report the relationship with their clinico-immunological features of their disease. Methods We recruited 126 subjects with a diagnosis of asthma, 94% with moderate-severe disease, and 18 healthy volunteers. At a single stable visit, subjects underwent spirometry; sputum fungal culture and a sputum cell differential count; skin prick testing to both common aeroallergens and an extended fungal panel; specific IgE to Aspergillus fumigatus. Fungi were identified by morphology and species identity was confirmed by sequencing. Four patients had allergic bronchopulmonary aspergillosis. Results Forty-eight percent of asthma subjects were IgE-sensitized to one fungal allergen and 22% to ≥ 2. Twenty-seven different taxa of filamentous fungi were isolated from 54% of their sputa, more than one species being detected in 17%. This compared with 3 (17%) healthy controls culturing any fungus (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|