Cloning and characterization of a mosquito larvicidal toxin produced during vegetative stage of Bacillus sphaericus 2297
| Autor: | Patcharee Promdonkoy, Sakol Panyim, Namchai Chewawiwat, Mongkon Audtho, Sutipa Tanapongpipat, Plearnpis Luxananil, Boonhiang Promdonkoy |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Insecticides Recombinant Fusion Proteins Bacterial Toxins Molecular Sequence Data Enterobacter Bacillus Aedes aegypti Protein Sorting Signals Applied Microbiology and Biotechnology Microbiology Bacillus sphaericus Open Reading Frames Bacterial Proteins Anopheles dirus parasitic diseases Escherichia coli Animals Cloning Molecular Promoter Regions Genetic Gene Glutathione Transferase Cloning biology fungi General Medicine Gene Expression Regulation Bacterial Sequence Analysis DNA biology.organism_classification Fusion protein Molecular biology Culex quinquefasciatus Recombinant Proteins Open reading frame Culicidae Larva |
| Zdroj: | Current microbiology. 49(2) |
| ISSN: | 0343-8651 |
| Popis: | The mosquitocidal toxin 1 (mtx1) gene from genomic DNA of B. sphaericus strain 2297 was cloned and expressed in E. coli. DNA sequencing analysis of the cloned gene revealed a single open reading frame encoding an 870-amino acid polypeptide. Expression level of the full-length gene in E. coli was very low even though strong promoter was used or the gene was expressed as a fusion protein. Expression level was highly improved after the putative leader sequence was deleted, and the truncated gene was expressed as a fusion protein with glutathione S-transferase (GST-tMtx1). E. coli cells expressing GST-tMtx1 was highly toxic to Culex quinquefasciatus larvae and showed lower toxicity against Anopheles dirus and Aedes aegypti larvae. Enterobacter amnigenus An11, a mosquito larval gut colonizable bacteria, transformed with the cloned gene exhibited mosquito larvicidal activity. Result suggested that there is a potential to develop this protein to be used as an alternative mosquito control agent. |
| Databáze: | OpenAIRE |
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