Halothane alters electrical activity and calcium dynamics in cultured mouse cortical, spinal cord, and dorsal root ganglion neurons
| Autor: | John J. Franks, W. T. Franks, Artur W. Wamil, Jean-Louis Horn, Piotr K. Janicki |
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| Rok vydání: | 1996 |
| Předmět: |
Central nervous system
Calcium-Transporting ATPases Tetrodotoxin Mice Dorsal root ganglion Ganglia Spinal medicine Animals Repolarization Fluorescent Antibody Technique Indirect Cells Cultured Fluorescent Dyes Cerebral Cortex Neurons Chemistry General Neuroscience Depolarization Microfluorimetry Spinal cord Electrophysiology medicine.anatomical_structure Spinal Cord Anesthetics Inhalation Biophysics Eosine Yellowish-(YS) Calcium Capsaicin Halothane Ion Channel Gating Microelectrodes Neuroscience medicine.drug |
| Zdroj: | Neuroscience Letters. 216:93-96 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/0304-3940(96)13003-2 |
| Popis: | Halothane inhibits neural plasma membrane Ca(2+)-ATPase, a pump that ejects Ca2+ from the cell after influx through voltage- or ligand-activated channels. Intracellular microelectrode recordings in mouse embryonic cortical and spinal cord neurons showed that halothane and eosin, a pump inhibitor, prolonged repolarization associated with spontaneous bursts of depolarization. These agents also prolonged the repolarization phases of electrically induced action potentials and of capsaicin-mediated Ca(2+)-dependent depolarization in mouse adult dorsal root ganglion neurons. In keeping with these findings, confocal microfluorimetry showed that halothane delayed clearance of intracellular Ca2+ accumulated by N-methyl-D-aspartate stimulation of single neurons. |
| Databáze: | OpenAIRE |
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