High prevalence of genotype 6 hepatitis C virus infection in Southern Taiwan using Abbott genotype assays
| Autor: | Li-Ching Wu, Hung-Da Tung, Jyh-Jou Chen, Na-Mi Lu, Ming-Jen Sheu, Chuan Lee, Hsing-Tao Kuo, Pei-Lun Lee, Hsu-Ju Kao, Tang-Wei Chuang, Chun-Ta Cheng |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Genotype Hepatitis C virus Southern taiwan Taiwan Viremia Hepacivirus Viral Nonstructural Proteins Real-Time Polymerase Chain Reaction medicine.disease_cause Antiviral Agents Southeast asia 03 medical and health sciences 0302 clinical medicine Prevalence Humans Medicine Phylogeny Aged lcsh:R5-920 High prevalence business.industry Endemic area Sequence Analysis DNA General Medicine Hepatitis C Middle Aged medicine.disease Virology 030220 oncology & carcinogenesis Female 030211 gastroenterology & hepatology 5' Untranslated Regions lcsh:Medicine (General) business |
| Zdroj: | Journal of the Formosan Medical Association, Vol 119, Iss 1, Pp 413-419 (2020) |
| ISSN: | 0929-6646 |
| Popis: | Background/Purpose: Abbott RealTime Genotype II assay can effectively identify hepatitis C virus (HCV) genotypes (GTs), but some GT 6 subtypes might not be differentiated from GT 1. Abbott RealTime Genotype II PLUS and sequencing might be needed to resolve these ambiguous results. Unlike the high prevalence of GT 6 in Southeast Asia, GT 6 had rarely been reported in Taiwan except in intravenous drug abusers (IDU). But the prevalence of GT 6 in Taiwan might be underestimated. We conducted this study to determine the GTs in a HCV endemic area in Southern Taiwan. Methods: A total of 1147 patients with hepatitis C viremia for direct acting antivirals (DAA) treatment at the Chi Mei medical system in Tainan were enrolled. Genotype was determined using a working flow consisted of Abbott GT II, PLUS assays and 5′ untranslated region (5′ UTR)/core sequencing. Results: Among the 1147 patients, 883 (77.0%) obtained GT results by GT II, 264 (23.0%) samples with ambiguous results by GT II assay received further tests, including 194 (73.5%) with PLUS assay and 70 (26.5%) with 5′UTR/core sequencing. Nearly three-quarters (73.5%) of ambiguous results by GT II assay were GT 6. Overall, 18.3% of samples were GT 6. Phylogenetic study of 11 samples of GT 6 subtypes showed 7 (63.6%) were 6 g. Conclusion: GT 6 is the major factor for high ambiguous rate by GT II. Unexpected high prevalence of GT 6 (18.3%) in Southern Taiwan, especially subtype 6 g, closely related to Indonesian strains, is first reported. Keywords: Genotype 6, Hepatitis C, Genotype assay, Direct acting antiviral |
| Databáze: | OpenAIRE |
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