Evaluation of clinical effectiveness of paclitaxel and ursolic acid co-loaded liposomes as enhanced treatment for head and neck squamous cell carcinoma
| Autor: | Ling Zhang, Xuejun Shang, Bi-Hua Lv, Wei Tan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
endocrine system
Chemotherapy Liposome medicine.medical_treatment Pharmaceutical Science Cancer Combination chemotherapy 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease complex mixtures Head and neck squamous-cell carcinoma 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Paclitaxel chemistry Ursolic acid 030220 oncology & carcinogenesis Cancer research medicine Pharmacology (medical) MTT assay 0210 nano-technology Paclitaxel Ursolic acid Combination chemotherapy Head-and-neck squamous cancer |
| Zdroj: | Tropical Journal of Pharmaceutical Research; Vol 17, No 11 (2018); 2115-2121 |
| ISSN: | 1596-9827 1596-5996 |
| DOI: | 10.4314/tjpr.v17i11.1 |
| Popis: | Purpose: To enhance the clinical effectiveness of paclitaxel (PTX) by co-delivery with ursolic acid (UA) for the treatment of head and neck cancer. Methods: Co-loaded liposomes of PTX and UA (UA-PTX-LiP) were prepared by thin-film hydration method. Their size and loading efficiency were determined using dynamic light scattering (DLS) technique and high performance liquid chromatography (HPLC), respectively. The effectiveness of UAPTX-LiP against HSC-3 human head and neck cancer cell-lines was compared with that of PTX liposome (PTX-LiP) using systemic cell-based in vitro evaluation with MTT assay. Fluorescent microscopy was used for cell uptake studies. Results: The size of the prepared UA-PTX-LiP was 126.5 ± 3.22 nm. The ratiometric system for PTX and UA as liposom es revealed significantly enhanced cytotoxicity, with comparatively lower IC 50 , whencompared to individual PTX-Lip. Fluorescent microscopy revealed the internalization ability of UA-PTXLiP by targeted delivery of PTX in HSC-3 human head and neck cancer cell-line. Conclusion: These results show that UA-PTX-LiP successfully enhances the therapeutic potential and clinical outcomes of PTX in head-and-neck cancer, and also demonstrate the useful effect of combination of UA and PTX in chemotherapy. Keywords: Paclitaxel, Ursolic acid, Combination chemotherapy, Head-and-neck squamous cancer |
| Databáze: | OpenAIRE |
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