Ectopic nuclear reorganisation driven by a Hoxb1 transgene transposed into Hoxd
| Autor: | Marie Kmita, Nelly R. Da Silva, Denis Duboule, Wendy A. Bickmore, Céline Morey |
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| Předmět: |
Limb Buds
Primitive Streak Transgene Cell Differentiation/genetics Rhombomere Embryonic Development Mice Transgenic Biology Article Transgenic Primitive Streak/embryology/metabolism/ultrastructure Homeodomain Proteins/*genetics Mice medicine Animals Rhombencephalon/embryology/metabolism/ultrastructure Transgenes Hox gene Body Patterning Cell Nucleus/*genetics/ultrastructure Cell Nucleus Homeodomain Proteins Regulation of gene expression Genetics Limb Buds/embryology/metabolism/ultrastructure Primitive streak Chromatin/genetics Chromatin Assembly and Disassembly/genetics Embryonic Development/*genetics Genes Homeobox Homeobox/genetics Gene Expression Regulation Developmental Cell Differentiation Cell Biology Chromatin Assembly and Disassembly Body Patterning/*genetics Chromatin Rhombencephalon DNA Transposable Elements/genetics Cell nucleus medicine.anatomical_structure Developmental/genetics Gene Expression Regulation Genes Transgenes/*genetics DNA Transposable Elements Homeobox |
| Zdroj: | Morey, C, Da Silva, N R, Kmita, M, Duboule, D & Bickmore, W A 2008, ' Ectopic nuclear reorganisation driven by a Hoxb1 transgene transposed into Hoxd ', Journal of Cell Science, vol. 121, no. Pt 5, pp. 571-7 . https://doi.org/10.1242/jcs.023234 |
| DOI: | 10.1242/jcs.023234 |
| Popis: | The extent to which the nuclear organisation of a gene impacts on its ability to be expressed, or whether nuclear organisation merely reflects gene expression states, remains an important but unresolved issue. A model system that has been instrumental in investigating this question utilises the murine Hox gene clusters encoding homeobox-containing proteins. Nuclear reorganisation and chromatin decondensation, initiated towards the 3′ end of the clusters, accompanies activation of Hox genes in both differentiation and development, and might be linked to mechanisms underlying colinearity. To investigate this, and to delineate the cis-acting elements involved, here we analyse the nuclear behaviour of a 3′ Hoxb1 transgene transposed to the 5′ end of the Hoxd cluster. We show that this transgene contains the cis-acting elements sufficient to initiate ectopic local nuclear reorganisation and chromatin decondensation and to break Hoxd colinearity in the primitive streak region of the early embryo. Significantly, in rhombomere 4, the transgene is able to induce attenuated nuclear reorganisation and decondensation of Hoxd even though there is no detectable expression of the transgene at this site. This shows that reorganisation of chromosome territories and chromatin decondensation can be uncoupled from transcription itself and suggests that they can therefore operate upstream of gene expression. |
| Databáze: | OpenAIRE |
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