‘Off-the-Shelf’ Immunotherapy: Manufacture of CD8+ T Cells Derived from Hematopoietic Stem Cells
| Autor: | Richard L. Boyd, Nicholas Boyd, Vera Evtimov, Aleta Pupovac, Kellie Cartledge, Alan O Trounson, Huimin Cao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cytotoxicity
Immunologic QH301-705.5 medicine.medical_treatment T cell Biology CD8-Positive T-Lymphocytes HSC off-the-shelf immunotherapy CD8+ T cells Article Immune system Cancer immunotherapy Cell Line Tumor medicine Cytotoxic T cell Animals Humans Biology (General) Cell Proliferation CD28 Cell Differentiation General Medicine Immunotherapy differentiation Fetal Blood Hematopoietic Stem Cells medicine.anatomical_structure Phenotype Cancer research Cattle Stem cell CD8 |
| Zdroj: | Cells Volume 10 Issue 10 Cells, Vol 10, Iss 2631, p 2631 (2021) |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells10102631 |
| Popis: | Cellular immunotherapy is revolutionizing cancer treatment. However, autologous transplants are complex, costly, and limited by the number and quality of T cells that can be isolated from and expanded for re-infusion into each patient. This paper demonstrates a stromal support cell-free in vitro method for the differentiation of T cells from umbilical cord blood hematopoietic stem cells (HSCs). For each single HSC cell input, approximately 5 × 104 T cells were created with an initial five days of HSC expansion and subsequent T cell differentiation over 49 days. When the induced in vitro differentiated T cells were activated by cytokines and anti-CD3/CD28 beads, CD8+ T cell receptor (TCR) γδ+ T cells were preferentially generated and elicited cytotoxic function against ovarian cancer cells in vitro. This process of inducing de novo functional T cells offers a possible strategy to increase T cell yields, simplify manufacturing, and reduce costs with application potential for conversion into chimeric antigen receptor (CAR)-T cells for cancer immunotherapy and for allogeneic transplantation to restore immune competence. |
| Databáze: | OpenAIRE |
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