Gene expression profiling during asexual development of the late blight pathogen Phytophthora infestans reveals a highly dynamic transcriptome
| Autor: | Luis da Cunha, Laura J. Grenville-Briggs, Paul R. J. Birch, John David Windass, Audrey M. V. Ah-Fong, Sophien Kamoun, Pieter van West, Wilco Ligterink, Samuel Roberts, Carrie S Thurber, Cahid Cakir, Howard S. Judelson, Stephen C. Whisson, Francine Govers, Maita Latijnhouwers, Anna O. Avrova, Harold J. G. Meijer, George Aux, Catherine R. Bruce |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Phytophthora
family sporulation Transcription Genetic Physiology rt-pcr cyst germination protein-kinase Models Biological Transcriptome Gene expression rna zoospore motility Gene parallel analysis Oligonucleotide Array Sequence Analysis Regulator gene Oomycete Genetics biology Reverse Transcriptase Polymerase Chain Reaction Effector EPS-2 Gene Expression Profiling fungi food and beverages General Medicine biology.organism_classification Laboratorium voor Phytopathologie PRI Bioscience potato infection Phytophthora infestans Laboratory of Phytopathology Agronomy and Crop Science host-plant cells |
| Zdroj: | Molecular Plant-Microbe Interactions, 21(4), 433-447 Molecular Plant-Microbe Interactions 21 (2008) 4 |
| ISSN: | 0894-0282 |
| Popis: | Much of the pathogenic success of Phytophthora infestans, the potato and tomato late blight agent, relies on its ability to generate from mycelia large amounts of sporangia, which release zoospores that encyst and form infection structures. To better understand these stages, Affymetrix GeneChips based on 15,650 unigenes were designed and used to profile the life cycle. Approximately half of P. infestans genes were found to exhibit significant differential expression between developmental transitions, with approximately 1/10 being stage-specific and most changes occurring during zoosporogenesis. Quantitative reverse-transcription polymerase chain reaction assays confirmed the robustness of the array results and showed that similar patterns of differential expression were obtained regardless of whether hyphae were from laboratory media or infected tomato. Differentially expressed genes encode potential cellular regulators, especially protein kinases; metabolic enzymes such as those involved in glycolysis, gluconeogenesis, or the biosynthesis of amino acids or lipids; regulators of DNA synthesis; structural proteins, including predicted flagellar proteins; and pathogenicity factors, including cell-wall-degrading enzymes, RXLR effector proteins, and enzymes protecting against plant defense responses. Curiously, some stage-specific transcripts do not appear to encode functional proteins. These findings reveal many new aspects of oomycete biology, as well as potential targets for crop protection chemicals. |
| Databáze: | OpenAIRE |
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