Isolation and Initial Characterization of a Novel Zinc Finger Gene, DNMT3L, on 21q22.3, Related to the Cytosine-5- Methyltransferase 3 Gene Family
| Autor: | Kazunori Shibuya, Juha Ollila, Jun Kudoh, Ai Shintani, Maarit Heino, Nobuyoshi Shimizu, Mauno Vihinen, Hamish S. Scott, Pärt Peterson, Shinsei Minoshima, Ulla Aapola, Kai Krohn, Stylianos E. Antonarakis, Kazuhiko Kawasaki |
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| Rok vydání: | 2000 |
| Předmět: |
Models
Molecular Protein family Chromosomes Human Pair 21 Protein Conformation Zinc Fingers/ genetics Molecular Sequence Data Sequence alignment Biology DNA Methyltransferase 3A Mice 03 medical and health sciences 0302 clinical medicine Genetics Animals Humans Gene family DNA (Cytosine-5-)-Methyltransferases Amino Acid Sequence Northern blot DNA (Cytosine-5-)-Methyltransferase/chemistry/ genetics/metabolism Peptide sequence Gene ATRX 030304 developmental biology ddc:616 Zinc finger 0303 health sciences Chromosome Mapping Zinc Fingers Molecular biology Chromosomes Human Pair 21/ genetics Protein Structure Tertiary 030220 oncology & carcinogenesis Sequence Alignment |
| Zdroj: | Genomics, Vol. 65, No 3 (2000) pp. 293-298 |
| ISSN: | 0888-7543 |
| DOI: | 10.1006/geno.2000.6168 |
| Popis: | We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family. The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. |
| Databáze: | OpenAIRE |
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