Downregulation of ABCD1 in Human Renal Cell Carcinoma
| Autor: | Yeong-Shiau Pu, Wen-Jeng Wu, Guang-Yan Liu, Wang-Yi Kang, Yi-Zih Kuo, Yu-Chieh Tsai, Chao-Yuan Huang, Tzyh-Chyuan Hour, Shu-Pin Huang |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty Cancer Research 040301 veterinary sciences Clinical Biochemistry Down-Regulation Biology medicine.disease_cause urologic and male genital diseases ATP Binding Cassette Transporter Subfamily D Member 1 Polymerase Chain Reaction 030226 pharmacology & pharmacy Metastasis Pathology and Forensic Medicine 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Renal cell carcinoma Cell Line Tumor medicine Carcinoma Humans RNA Messenger Carcinoma Renal Cell Neoplasm Staging Kidney Reverse Transcriptase Polymerase Chain Reaction Cancer 04 agricultural and veterinary sciences medicine.disease Immunohistochemistry Kidney Neoplasms medicine.anatomical_structure Kidney Tubules Oncology ATP-Binding Cassette Transporters Female Carcinogenesis Kidney cancer |
| Zdroj: | The International Journal of Biological Markers. 24:171-178 |
| ISSN: | 1724-6008 |
| Popis: | Renal cell carcinoma (RCC) is the most common malignant tumor of the kidney. Delayed diagnosis may result in progression and metastasis. Markers for early detection of RCC are lacking. The ATP-binding cassette transporter D1 (ABCD1) is located in the human peroxisome membrane. Its mutation causes X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder affecting lipid storage. The role of ABCD1 in human renal tumorigenesis was unclear. In this study, three pairs of RCC tissues were examined by cDNA microarray and data suggested that ABCD1 mRNA is downregulated. Downregulation of ABCD1 expression was confirmed by real-time PCR. ABCD1 expression was also downregulated in four renal cancer cell lines compared to immortalized benign renal tubular cells. ABCD1 mRNA and protein expression levels assessed by immunohistochemistry in the RCC tissues were similar between genders, tumor grades, and tumor stages. Immunohisto-chemical assays also showed that ABCD1 expression was significantly higher in normal than in cancerous tissues (p |
| Databáze: | OpenAIRE |
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