Synchronizing developmental stages in Neotropical catfishes for application in germ cell transplantation
| Autor: | Nycolas Levy-Pereira, Leonardo Luiz Calado, Paulo Sérgio Monzani, Nivaldo Ferreira do Nascimento, Diógenes Henrique de Siqueira-Silva, George Shigueki Yasui, Matheus Pereira dos Santos, Dilberto Ribeiro Arashiro, Silvio Carlos Alves do Santos, José Augusto Senhorini |
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| Přispěvatelé: | Chico Mendes Inst Biodivers Conservat, Universidade Estadual Paulista (Unesp), AES Tiete, FZEA, UNIFESSPA Fed Univ South Southeast Para |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Embryo Nonmammalian food.ingredient Embryonic Development Biology Surrogate propagation 03 medical and health sciences food Animal science Human fertilization Yolk Siluridae Animals Incubation Catfishes Cell Size Hatching Endangered Species Embryogenesis PGC Temperature Embryo Cell Biology Blastula biology.organism_classification Transplantation 030104 developmental biology Fertilization Larva Oocytes Female Developmental Biology |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199418000035 |
| Popis: | Made available in DSpace on 2018-11-26T17:54:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 AES Tiete The aim of this study was to describe the effect of temperature on the fertilization, early developmental stages, and survival rate of two Neotropical catfishes Pimelodus maculatus and Pseudopimelodus mangurus. After fertilization, the eggs were incubated at 22 degrees C, 26 degrees C, and 30 degrees C, which resulted in fertilization rates of 96.95 +/- 1.79%, 98.74 +/- 0.76%, and 98.44 +/- 0.19% for P. maculatus and 96.10 +/- 1.58%, 98.00 +/- 0.63%, and 94.60 +/- 2.09% for P. mangurus, respectively. For P. maculatus, hatching occurred after 22 h 30 min post-fertilization at 22 degrees C, 16 h 30 min at 26 degrees C, and 11 h 20 min at 30 degrees C, and the hatching rates were 43.87 +/- 7,46%, 57.57 +/- 17.49%, and 53.63 +/- 16.27%, respectively. For P. mangurus, hatching occurred after 28 h 30 min post-fertilization at 22 degrees C and 17 h 30 min at 26 degrees C with respective hatching rates of 45.4 +/- 21.02% and 68.1 +/- 12.67%. For this species, all embryos incubated at 30 degrees C died before hatching. Additionally, for P. maculatus, the larvae from the lower (22 degrees C) and higher temperatures (30 degrees C) presented increased abnormality rates, as observed in the head, tail and yolk regions. The lowest abnormality rate was detected at 26 degrees C, which was considered the optimal incubation temperature for both species. The developed protocol enables the manipulation of embryonic development, which is important for the application of reproductive biotechniques, including chimerism and chromosome-set manipulation. The data obtained here are also important for the surrogate propagation of this species as P. mangurus was recently categorized as an endangered fish species. Chico Mendes Inst Biodivers Conservat, Natl Ctr Res Conservat Continental Fish, Lab Fish Biotechnol, Rodovia Pref Euberto Nemesio Pereira de Godoy, Pirassununga, Brazil Sao Paulo State Univ, Inst Biosci, Rua Prof Doutor Antonio Celso Wagner Zanin S-No, BR-18618689 Botucatu, SP, Brazil Sao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil AES Tiete, Br-153,Rod,0 Km 139 Ctr, BR-16370000 AES Tiete, SP, Brazil FZEA, Dept Vet Med, Ave Duque Caxias Norte 225, BR-13639080 Pirassununga, SP, Brazil UNIFESSPA Fed Univ South Southeast Para, Inst Hlth Biol Studies IESB, Folha 31,Quadra 7,Lote Especial S-N, BR-68507590 Maraba, PA, Brazil Sao Paulo State Univ, Inst Biosci, Rua Prof Doutor Antonio Celso Wagner Zanin S-No, BR-18618689 Botucatu, SP, Brazil Sao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil |
| Databáze: | OpenAIRE |
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