Pax-5 Inhibits Breast Cancer Proliferation Through MiR-215 Up-regulation
| Autor: | Nicolas LeBlanc, Gilles A. Robichaud, Rodney J. Ouellette, Jason Harquail, Nicolas Crapoulet |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Breast Neoplasms Biology law.invention 03 medical and health sciences Breast cancer Downregulation and upregulation law Cell Movement Cell Line Tumor microRNA medicine Humans Gene Cell Proliferation Antitumor activity Cell invasion Cell growth Sequence Analysis RNA Gene Expression Profiling PAX5 Transcription Factor High-Throughput Nucleotide Sequencing General Medicine medicine.disease Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Oncology embryonic structures Cancer research Suppressor Female |
| Zdroj: | Anticancer research. 38(9) |
| ISSN: | 1791-7530 |
| Popis: | Background/aim In breast cancer, Pax-5 promotes pro-epithelial features and suppresses malignant cancer processes. However, the molecular mechanism of this antitumor activity remains largely unknown. This study aimed to identify the cellular roles of Pax-5-regulated miRNAs in breast cancer progression. Materials and methods After transient transfection of Pax-5 in MDA-MB-231 breast cancer cells, Pax-5-regulated miRNA expression was examined by next-generation sequencing. The identified Pax-5-regulated miRNAs were then validated by qRT-PCR and examined for the roles they play in breast cancer cells. Results Pax-5 was shown to be an effective modulator of miR-215-5p and its target genes. MiR-215 inhibited cell proliferation and migration of breast cancer cells, but not cell invasion. More importantly, Pax-5-induced suppression of cancer cell proliferation and migration was found to be miR-215-dependent. Interestingly, miR-215 profiling in clinical tumor samples showed that miR-215 expression was lower in cancer tissues in comparison to healthy controls. Conclusion Pax-5 reduces breast cancer proliferation and migration through up-regulation of the tumor suppressor miR-215. This result supports the use of miR-215 as a prognostic marker for breast cancer. |
| Databáze: | OpenAIRE |
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