Gomisin J from Schisandra chinensis induces vascular relaxation via activation of endothelial nitric oxide synthase
| Autor: | Seung Jin Lee, Jung Wook Yun, Young-Whan Choi, Chi Dae Kim, So Youn Park, Kyo Won Seo, Jin Ung Bae, Ji Young Park |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Time Factors Nitric Oxide Synthase Type III Endothelium Physiology chemistry.chemical_element Aorta Thoracic Calcium Pharmacology Nitric Oxide Endothelial NOS Lignans Calcium in biology Nitric oxide Rats Sprague-Dawley Phosphatidylinositol 3-Kinases chemistry.chemical_compound Enos medicine Animals Polycyclic Compounds Phosphorylation Schisandra Dose-Response Relationship Drug biology Endothelial Cells biology.organism_classification Coronary Vessels Rats Vasodilation Nitric oxide synthase EGTA medicine.anatomical_structure chemistry Biochemistry biology.protein Molecular Medicine Proto-Oncogene Proteins c-akt |
| Zdroj: | Vascular Pharmacology. 57:124-130 |
| ISSN: | 1537-1891 |
| Popis: | Gomisin J (GJ) is a lignan contained in Schisandra chinensis (SC) which is a well-known medicinal herb for improvement of cardiovascular symptoms in Korean. Thus, the present study examined the vascular effects of GJ, and also determined the mechanisms involved. Exposure of rat thoracic aorta to GJ (1-30μg/ml) resulted in a concentration-dependent vasorelaxation, which was more prominent in the endothelium (ED)-intact aorta. ED-dependent relaxation induced by GJ was markedly attenuated by pretreatment with L-NAME, a nitric oxide synthase (NOS) inhibitor. In the intact endothelial cells of rat thoracic aorta, GJ also enhanced nitric oxide (NO) production. In studies using human coronary artery endothelial cells, GJ enhanced phosphorylation of endothelial NOS (eNOS) at Ser(1177) with increased cytosolic translocation of eNOS, and subsequently increased NO production. These effects of GJ were attenuated not only by calcium chelators including EGTA and BAPTA-AM, but also by LY294002, a PI3K/Akt inhibitor, indicating calcium- and PI3K/Akt-dependent activation of eNOS by GJ. Moreover, the levels of intracellular calcium were increased immediately after GJ administration, but Akt phosphorylation was started to increase at 20min of GJ treatment. Based on these results with the facts that ED-dependent relaxation occurred rapidly after GJ treatment, it was suggested that the ED-dependent vasorelaxant effects of GJ were mediated mainly by calcium-dependent activation of eNOS with subsequent production of endothelial NO. |
| Databáze: | OpenAIRE |
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