Germination of Bacillus cereus spores adhered to stainless steel
| Autor: | Roy Moezelaar, P.P.L.A. de Leeuw, E.J.H. Wolbert, W.M. de Vos, Luc M. Hornstra, Y.P. de Vries, Tjakko Abee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
sporulation
Bacillus cereus l-alanine Food Contamination Endospore Microbiology Bacterial Adhesion Levensmiddelenmicrobiologie resistance Hand sanitizer Microbiologie Spore germination Food microbiology surface Food science inactivation VLAG hydrophobicity Spores Bacterial milk Alanine AFSG Food Quality biology removal fungi Hygiene General Medicine biology.organism_classification Stainless Steel Inosine Spore adhesion sanitizer Cereus Germination AFSG Biobased Products Food Microbiology Equipment Contamination Food Science |
| Zdroj: | International Journal of Food Microbiology, 116(3), 367-371 International Journal of Food Microbiology 116 (2007) 3 |
| ISSN: | 0168-1605 |
| DOI: | 10.1016/j.ijfoodmicro.2007.02.012 |
| Popis: | Adhered spores of Bacillus cereus represent a significant part of the surface-derived contamination in processing equipment used in the dairy industry. As germinated spores lose their resistance capacities instantaneously, efficient germination prior to a cleaning in place treatment could aid to the disinfecting effect of such a treatment. Therefore, spores of B. cereus ATCC 14579 and that of the environmental isolate B. cereus CMCC 3328 were assessed for their germination behaviour when adhered to a stainless steel surface. A mixture of l-alanine and inosine initiated germination of adhered spores efficiently, resulting in 3.2 decimal logarithms of germination. Notably, implementation of a germination-inducing step prior to a representative cleaning in place procedure reduced the number of survivors with over 3 decimal log units, while an alkali treatment alone, as part of the cleaning in place procedure, did not show any effect on B. cereus spore viability. These results show that implementation of a germination step enhances the disinfection effect of currently used cleaning in place procedures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect