Cloning and Disruption of pgx4 Encoding an In Planta Expressed Exopolygalacturonase from Fusarium oxysporum
Autor: | Fe I. García-Maceira, M.I.G. Roncero, A. di Pietro |
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Rok vydání: | 2000 |
Předmět: |
Glycoside Hydrolases
Physiology Genes Fungal Molecular Sequence Data Microbiology Fungal Proteins Fusarium Solanum lycopersicum Fusarium oxysporum Cochliobolus carbonum Amino Acid Sequence Cloning Molecular Gene Peptide sequence chemistry.chemical_classification Fungal protein Sequence Homology Amino Acid biology Reverse Transcriptase Polymerase Chain Reaction Fungal genetics Nucleic acid sequence food and beverages General Medicine Blotting Northern biology.organism_classification Amino acid Blotting Southern chemistry Mutagenesis Agronomy and Crop Science |
Zdroj: | Molecular Plant-Microbe Interactions®. 13:359-365 |
ISSN: | 1943-7706 0894-0282 |
Popis: | Fusarium oxysporum f. sp. lycopersici, the causal agent of tomato vascular wilt, produces an array of pectinolytic enzymes, including at least two exo-α1,4-polygalac-turonases (exoPGs). A gene encoding an exoPG, pgx4, was isolated with degenerate polymerase chain reaction primers derived from amino acid sequences conserved in two fungal exoPGs. pgx4 encodes a 454 amino acid polypeptide with nine potential N-glycosylation sites and a putative 21 amino acid N-terminal signal peptide. The deduced mature protein has a calculated molecular mass of 47.9 kDa, a pI of 8.0, and 51 and 49% identity with the exoPGs of Cochliobolus carbonum and Aspergillus tubingensis, respectively. The gene is present in a single copy in different formae speciales of F. oxysporum. Expression of pgx4 was detected during in vitro growth on pectin, polygalacturonic acid, and tomato vascular tissue and in roots and stems of tomato plants infected by F. oxysporum f. sp. lycopersici. Two mutants of F. oxy-sporum f. sp. lycopersici with a copy of pgx4 inactivated by gene replacement were as virulent on tomato plants as the wild-type strain. |
Databáze: | OpenAIRE |
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