A Novel Rind Puncture Technique to Measure Rind Thickness and Diameter in Plant Stalks
Autor: | Jadzia Graves, Will Seegmiller, Daniel J. Robertson |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Materials science Plant Science lcsh:Plant culture Penetration 01 natural sciences 03 medical and health sciences Diameter Lodging High throughput Genetics lcsh:SB1-1110 lcsh:QH301-705.5 030304 developmental biology 2. Zero hunger 0303 health sciences Stalk 010401 analytical chemistry Methodology Plant Rind 0104 chemical sciences Puncture Phenotype 030104 developmental biology lcsh:Biology (General) Calipers Strength Thickness 010606 plant biology & botany Biotechnology Biomedical engineering |
Zdroj: | Plant Methods, Vol 16, Iss 1, Pp 1-8 (2020) Plant Methods |
Popis: | Background Measurements of rind and culm thickness and stem radius/diameter are important to biomechanical, ecological and physiological plant studies. However, many methods of measuring rind thickness and diameter are labor intensive and induce plant fatality. A novel rind puncture methodology for obtaining measurements of rind thickness and diameter has been developed. The suitability of the new method for implementation in plant studies is presented. Results The novel rind puncture technique was used to obtain measurements of rind thickness and diameter for samples of Poison Hemlock (Conium maculatum). The rind puncture measurements were strongly correlated with caliper measurements (R2 > 0.97) and photographic image analysis measurements (R2 > 0.84). The capacity for high throughput measurements using the rind puncture technique was determined to exceed that of caliper measurements and image analysis techniques. Conclusions The rind puncture technique shows promise as a high throughput method for determining rind thickness and diameter as it is cost effective and non-lethal. The authors are currently working to develop a custom handheld apparatus to allow the novel rind puncture method to be used in field work. High throughput field-based measurements of rind thickness and diameter are needed to help address the problem of stalk lodging (failure of grain crops to remain upright until harvest). |
Databáze: | OpenAIRE |
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