Evaluation of a Semiquantitative Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Method for Rapid Antimicrobial Susceptibility Testing of Positive Blood Cultures
| Autor: | Jette Jung, Birgit Gross, Christoph Lange, Markus Kostrzewa, Sören Schubert, Christina Hamacher, Katrin Sparbier |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Cefotaxime Time Factors medicine.drug_class 030106 microbiology Antibiotics Microbial Sensitivity Tests Mass spectrometry 03 medical and health sciences Antibiotic resistance Drug Resistance Bacterial medicine Humans Blood culture Diagnostic Errors Chromatography biology medicine.diagnostic_test Chemistry Bacteriology biology.organism_classification Anti-Bacterial Agents Ciprofloxacin Blood Culture Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Gentamicin Bacteria medicine.drug |
| Zdroj: | Journal of clinical microbiology. 54(11) |
| ISSN: | 1098-660X |
| Popis: | With the increasing prevalence of multidrug-resistant Gram-negative bacteria, rapid identification of the pathogen and its individual antibiotic resistance is crucial to ensure adequate antiinfective treatment at the earliest time point. Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry for the identification of bacteria directly from the blood culture bottle has been widely established; however, there is still an urgent need for new methods that permit rapid resistance testing. Recently, a semiquantitative MALDI-TOF mass spectrometry-based method for the prediction of antibiotic resistance was described. We evaluated this method for detecting nonsusceptibility against two β-lactam and two non-β-lactam antibiotics. A collection of 30 spiked blood cultures was tested for nonsusceptibility against gentamicin and ciprofloxacin. Furthermore, 99 patient-derived blood cultures were tested for nonsusceptibility against cefotaxime, piperacillin-tazobactam, and ciprofloxacin in parallel with MALDI-TOF mass spectrometry identification from the blood culture fluid. The assay correctly classified all isolates tested for nonsusceptibility against gentamicin and cefotaxime. One misclassification for ciprofloxacin nonsusceptibility and five misclassifications for piperacillin-tazobactam nonsusceptibility occurred. Identification of the bacterium and prediction of nonsusceptibility was possible within approximately 4 h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|