Quantitation of PGE9509924, a novel, nonfluorinated quinolone, in rat plasma using liquid chromatography electrospray-tandem mass spectrometry following solid-phase extraction sample clean-up in a 96-well format
| Autor: | P.H. Zoutendam, M.K. Dirr, M.J. Martin, M. Gavin |
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| Rok vydání: | 2003 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray Chromatography Chemistry Clinical Biochemistry Extraction (chemistry) Pipette Analytical chemistry Reproducibility of Results Pharmaceutical Science Quinolones Tandem mass spectrometry High-performance liquid chromatography Rats Analytical Chemistry Drug Stability Drug Discovery Animals Adsorption Solid phase extraction Quantitative analysis (chemistry) Spectroscopy Chromatography Liquid Antibacterial agent |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 33:1073-1080 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/s0731-7085(03)00362-5 |
| Popis: | PGE9509924, a novel nonfluorinated quinolone, is a potent antibacterial agent with a broad spectrum of activity. A semi-automated method using 96-well format, solid-phase extraction has been developed for quantitating PGE9509924 in rat plasma. The Waters Oasis HLB extraction plate containing a polymeric packing material was found to give the best overall recoveries. All liquid transfer steps other than aliquoting the plasma are accomplished using a 96-channel pipettor. Reverse-phase HPLC with electrospray/MS/MS detection using selective reaction monitoring is used to quantitate the samples. Stable isotopically labeled PGE9509924 is used as the internal standard. The assay is linear over the range from 0.01 to 10 ug/ml. Excellent precision is obtained within a single run and between multiple runs performed on different days. CVs of |
| Databáze: | OpenAIRE |
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