Reduced Oxygen Concentration Enhances Conversion of Embryonic Stem Cells to Epiblast Stem Cells
| Autor: | Chiaki Hamanishi, Toshiyuki Takehara, Yuta Onodera, Kanji Fukuda, Takeshi Teramura |
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| Rok vydání: | 2012 |
| Předmět: |
STAT3 Transcription Factor
KOSR animal structures Transgene Green Fluorescent Proteins Mice Transgenic Embryoid body Biology Leukemia Inhibitory Factor Mice Animals Humans Embryonic Stem Cells Janus Kinases Embryo Cell Biology Hematology Anatomy Embryonic stem cell Cell Hypoxia Culture Media Cell biology Mice Inbred C57BL Oxygen Blastocyst Epiblast Mutation embryonic structures Limiting oxygen concentration Stem cell Octamer Transcription Factor-3 Germ Layers Signal Transduction Developmental Biology |
| Zdroj: | Stem Cells and Development. 21:1239-1249 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2011.0322 |
| Popis: | Recently, an additional type of pluripotent stem cell-line derived from mouse embryos has been established and termed epiblast stem cell (EpiSC), and is expected to be an important tool for studying the mechanisms of maintenance of pluripotency since they depend on basic fibroblast growth factor-MAPK and Activin A-Smad2/3 signaling to maintain pluripotency, unlike mouse embryonic stem cells (ESCs). Further, because of the similarities between mouse EpiSCs and human ESCs, EpiSCs are expected to be effective experimental models for human stem cell therapy. Recently, study for conversion from ESC state to EpiSC state or reversion from EpiSC state to ESC state has attracted interest since these techniques may lead to increasing the potential of pluripotent stem cells and our knowledge about their developmental status. In the present study, we find that a low oxygen concentration in culture environment accelerated, improved, and stabilized the EpiSC state of the converted cells from the ESC state using Oct4ΔPE-GFP transgenic ESCs. Induced EpiSCs (iEpiSCs) in hypoxia possess closer gene expression patterns to native EpiSCs, and bisulfite sequences for the promoter regions of Stella and Oct4 genes have elucidated that the iEpiSC gain EpiSC-specific methylation patterns in hypoxia. Our data provide evidence that oxygen concentration is an important factor for establishment of the EpiSC-specific state. |
| Databáze: | OpenAIRE |
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