MEG3 promotes proliferation and inhibits apoptosis in osteoarthritis chondrocytes by miR-361-5p/FOXO1 axis
| Autor: | Yefeng Zhang, Yong Shen, Yuanzhen Chen, Anying Wang, Yao Lv, Changhui Su, Naixia Hu |
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| Rok vydání: | 2019 |
| Předmět: |
Cartilage
Articular Male 0301 basic medicine lcsh:Internal medicine lcsh:QH426-470 Apoptosis Flow cytometry Extracellular matrix 03 medical and health sciences Chondrocytes 0302 clinical medicine Western blot Gene expression Osteoarthritis Genetics medicine Animals Humans lcsh:RC31-1245 Genetics (clinical) Cell Proliferation MEG3 Maternally expressed 3 miR-361-59 Base Sequence medicine.diagnostic_test Forkhead Box Protein O1 Cell growth Chemistry FOXO1 ECM degradation Transfection Rats Cell biology MicroRNAs lcsh:Genetics 030104 developmental biology 030220 oncology & carcinogenesis RNA Long Noncoding Research Article |
| Zdroj: | BMC Medical Genomics, Vol 12, Iss 1, Pp 1-11 (2019) BMC Medical Genomics |
| Popis: | Background This study aimed to investigate the role of long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) and related molecular mechanisms, in osteoarthritis (OA). Methods Cartilage tissues of OA patients and healthy volunteers were isolated and cultured. After transfection with the appropriate constructs, chondrocytes were classified into Blank, pcDNA3.1-NC, pcDNA3.1-MEG3, si-NC, si-MEG3, pcDNA3.1-NC + mimics NC, pcDNA3.1-MEG3 + mimics NC, pcDNA3.1-NC + miR-361-5p mimics and pcDNA3.1-MEG3 + miR-361-5p mimics groups. qRT-PCR was used to detect the expression of MEG3, miR-361-5p and FOXO1. Western blot, luciferase reporter assay, RIP, CCK-8, and flow cytometry analysis were performed to reveal the morphology, proliferation, and apoptotic status of cartilage cells. Histological analysis and immunostaining were conducted in the OA rat model. Results Expression of MEG3 and FOXO1 was significantly decreased in OA compared with the normal group, while the expression of miR-361-5p was increased. MEG3 might serve as a ceRNA of miR-361-5p in OA chondrocytes. Moreover, using western blot analyses and the CCK-8 assay, MEG3 was shown to target miR-361-5p/FOXO1, elevate cell proliferation, and impair cell apoptosis. Functional analysis in vivo showed that MEG3 suppressed degradation of the cartilage matrix. Conclusion MEG3 can contribute to cell proliferation and inhibit cell apoptosis and degradation of extracellular matrix (ECM) via the miR-361-5p/FOXO1 axis in OA chondrocytes. |
| Databáze: | OpenAIRE |
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