The LPS-induced neutrophil recruitment into rat air pouches is mediated by TNFα: likely macrophage origin
| Autor: | B. Boris Vargaftig, Arreto Cd, Dumarey C, M. Nahori |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Necrosis
Lipopolysaccharide business.industry Immunology Mepyramine Antagonist Cell Biology Cycloheximide Pharmacology chemistry.chemical_compound chemistry lcsh:Pathology Medicine Macrophage Tumor necrosis factor alpha medicine.symptom business Dexamethasone Research Article medicine.drug lcsh:RB1-214 |
| Zdroj: | Mediators of Inflammation, Vol 6, Iss 5-6, Pp 335-343 (1997) Mediators of Inflammation |
| ISSN: | 1466-1861 0962-9351 |
| Popis: | The role of resident cells during the lipopolysaccharide (LPS)-induced neutrophil recruitment into rat air pouches was investigated. In this model, LPS (Escherichia coli, O55: B5 strain; 2-2000 ng) induced a dose- and time-dependent neutrophil recruitment accompanied by the generation of a tumour necrosis factor-alpha (TNFalpha)-like activity. Dexamethasone (0.05-5 mug) and cycloheximide (6 ng), injected 2 h before LPS into the pouches, inhibited the neutrophil recruitment and the generation of the TNFalpha-like activity, while the H1-receptor antagonist mepyramine (1 and 4 mg/kg, i.p., 0.5 h before LPS) and the PAF-receptor antagonist WEB 2170 (0.05 and 1 mg/kg, i.p., 0.5 h before LPS) had no effect. Purified alveolar macrophages (AM) were used to replenish the pouches of cycloheximide-treated recipient rats. AM provided by PBS-treated animals led to the recovery of the LPS-induced neutrophil recruitment and of the TNFalpha-like formation contrasting with those from cycloheximide-treated animals (1 mg/kg, i.p.). When delivered in situ, liposome-encapsulated clodronate, a macrophage depletor, significantly impaired both the LPSinduced neutrophil recruitment and the TNFalpha-like activity. An anti-murine TNFalpha polyclonal antibody (0.5 h before LPS) was also effective. These results emphasize the pivotal role of macrophages for LPS-induced neutrophil recruitment via the formation of TNFalpha. |
| Databáze: | OpenAIRE |
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