Additional file 3: of Usability of reference-free transcriptome assemblies for detection of differential expression: a case study on Aethionema arabicum dimorphic seeds

Autor: Wilhelmsson, Per, Chandler, Jake, Fernandez-Pozo, Noe, Graeber, Kai, Ullrich, Kristian, Arshad, Waheed, Safina Khan, Hofberger, Johannes, Buchta, Karl, Edger, Patrick, J. Pires, M. Schranz, Leubner-Metzger, Gerhard, Rensing, Stefan
Rok vydání: 2019
DOI: 10.6084/m9.figshare.7654307
Popis: Figure S1. PCA of RPKM values for 6745 paired transcripts (identified in both genome and transcriptome methods) by method and morphotype. Figure S2. RPKM levels (reference genome approach) of the overlapping DEGs as well as of the non-overlapping DEGs called by NOISeq, edgeR and DESeq2. Figure S3. Expression of selected DEGs measured by qRT-PCR. Figure S4. showing abundances of Ae. arabicum ribonucleoprotein transcripts (a) and transcripts from selected gene categories (b) and Figure S5. showing the pattern of expression of select hormonal signaling related genes during A. thaliana seed maturation. Assessment of RNA integrity and purity (Figure S6.) and validation of reference genes used for qRT-PCR normalization (Figure S7.) (DOCX 2738 kb)
Databáze: OpenAIRE