Gel electrophoresis of polystyrene particles in glutaraldehyde crosslinked polyvinyl alcohol
| Autor: | Andreas Chrambach, Dietmar Tietz, T. Roy Ittyerah, David Halpern, Jan Pospichal |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Electrophoresis
Gel electrophoresis chemistry.chemical_classification Chemistry Clinical Biochemistry macromolecular substances Polymer Biochemistry Polyvinyl alcohol Microspheres Analytical Chemistry Molecular Weight chemistry.chemical_compound Cross-Linking Reagents Chemical engineering Glutaral Polyvinyl Alcohol Polymer chemistry Polystyrenes Agarose Fiber Polystyrene Glutaraldehyde Particle Size |
| Zdroj: | Electrophoresis. 12:338-341 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.1150120504 |
| Popis: | Polystyrene sulfate and carboxylate particles (19-189 nm radius) were subjected to electrophoresis in glutaraldehyde crosslinked polyvinyl alcohol of molecular weight 25.000 and 650.000 Da at various concentrations. The degree of crosslinking is severely limited by the mechanical properties of the gels that deteriorate beyond a glutaraldehyde concentration which decreases with increasing polyvinyl alcohol chain length. The effective fiber radius of the short-chain and long-chain polymer fiber was 45 +/- 25 and 131 +/- 47 nm, respectively. Thus, these media do not significantly exceed the apparent fiber thickness of agarose, are more difficult to prepare--but are well-defined synthetic products rather than natural ones, and have the advantage of carrying no net charge and can therefore be expected to exhibit no electroendosmosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text