Uneven distribution of Purkinje cell injury in the cerebellar vermis of term neonates with hypoxic-ischemic encephalopathy
| Autor: | Mjnl Benders, Floris Groenendaal, van der Aa N, Reint K. Jellema, Martin Lammens, Thomas Alderliesten, Jeroen Dudink, van Leeuwen I, Freek E. Hoebeek, Peter G. J. Nikkels, Nijboer C, Kim V. Annink |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty business.industry Purkinje cell Encephalopathy Autopsy Haematoxylin medicine.disease Hypoxic Ischemic Encephalopathy chemistry.chemical_compound medicine.anatomical_structure chemistry Cerebellar vermis Medicine Distribution (pharmacology) Animal studies business |
| DOI: | 10.1101/2020.04.15.043018 |
| Popis: | IntroductionIn term neonates with hypoxic-ischemic encephalopathy (HIE), cerebellar injury is becoming more and more acknowledged. Animal studies demonstrated that Purkinje cells (PCs) are especially vulnerable for hypoxic-ischemic injury. In neonates, however, the extent and pattern of PC injury has not been investigated. The aim of this study was to determine the distribution of PC injury in the cerebellar vermis of term born neonates with HIE.MethodsTerm born neonates with HIE that underwent post-mortem autopsy of the cerebellar vermis were included. Haematoxylin & Eosin (H&E) stained sections of the vermis were used to determine total PC count and morphology (normal, abnormal or non-classified) at the bases and crown of the folia and of the lobules in both the anterior and posterior lobes. Differences in PC count and PC morphology between the anterior and posterior lobe and between the bases and crown were calculated using the paired samples T-test or Wilcoxon-signed rank test.ResultsThe total number of PCs were significantly higher at the crown compared to the bases (pConclusionThe abnormal PC count and morphology in term neonates with HIE resembles supratentorial ulegyria. |
| Databáze: | OpenAIRE |
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