In Vitro Study of the Blood-Brain Barrier Transport of Natural Compounds Recovered from Agrifood By-Products and Microalgae
| Autor: | José David Sánchez-Martínez, Ana Rita Garcia, Gerardo Alvarez-Rivera, Alberto Valdés, Maria Alexandra Brito, Alejandro Cifuentes |
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| Přispěvatelé: | Ministerio de Educación y Formación Profesional (España), Ministerio de Ciencia e Innovación (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España) |
| Rok vydání: | 2022 |
| Předmět: |
Food waste
Organic Chemistry General Medicine neuroprotection blood–brain barrier green-extraction LC/GC-q-TOF-MS food waste bioactive compounds Catalysis Neuroprotection Bioactive compounds Computer Science Applications Inorganic Chemistry Blood–brain barrier Physical and Theoretical Chemistry Green-extraction Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 24; Issue 1; Pages: 533 |
| ISSN: | 1422-0067 |
| Popis: | This article belongs to the Special Issue The Role of Dietary Phytochemicals in Brain Health. Agrifood by-products and microalgae represent a low-cost and valuable source of bioactive compounds with neuroprotective properties. However, the neuroprotective effectiveness of therapeutic molecules can be limited by their capacity to cross the blood–brain barrier (BBB) and reach the brain. In this research, various green extracts from Robinia pseudoacacia (ASFE), Cyphomandra betacea (T33), Coffea arabica (PPC1), Olea europaea L., (OL-SS), Citrus sinensis (PLE100) by-products and from the microalgae Dunaliella salina (DS) that have demonstrated in vitro neuroprotective potential were submitted to an in vitro BBB permeability and transport assay based on an immortalized human brain microvascular endothelial cells (HBMEC) model. Toxicity and BBB integrity tests were performed, and the transport of target bioactive molecules across the BBB were evaluated after 2 and 4 h of incubation using gas and liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (GC/LC-Q-TOF-MS). The HBMEC-BBB transport assay revealed a high permeability of representative neuroprotective compounds, such as mono- and sesquiterpenoids, phytosterols and some phenolic compounds. The obtained results from the proposed in vitro BBB cellular model provide further evidence of the neuroprotective potential of the target natural extracts, which represent a promising source of functional ingredients to be transferred into food supplements, food additives, or nutraceuticals with scientifically supported neuroprotective claims. J.D.S.-M. would like to acknowledge the Ministry of Education and Vocational Training for a FPU predoctoral grant FPU17/01876. G.Á.-R. and A.V. would like to acknowledge the Ministry of Science and Innovation (MICINN) for their “Juan de la Cierva-Incorporación” postdoctoral grants IJC2019-041482-I and IJC2018-037560-I, respectively. This work was supported by the Ministry of Science and Innovation of Spain (Grant No. PID2020-113050RB-I00 and Grant No. PDC2021-120814-I00). |
| Databáze: | OpenAIRE |
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