Proprotein convertase subtilisin/kexin type 9 (PCSK9) Deficiency is Protective Against Venous Thrombosis in Mice
| Autor: | Jintao Wang, Daniel T. Eitzman, Chiao Guo, He Meng, Hui Wang, Jason S. Knight, Kyle Kleiman, Qian Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Science 030204 cardiovascular system & hematology Inferior vena cava Article 03 medical and health sciences Mice 0302 clinical medicine Internal medicine medicine Animals Subtilisins Thrombus Mice Knockout Venous Thrombosis Multidisciplinary business.industry PCSK9 Cholesterol HDL Serine Endopeptidases Cholesterol LDL medicine.disease Lipid Metabolism Thrombosis Lipids Mice Inbred C57BL Venous thrombosis 030104 developmental biology Endocrinology medicine.vein cardiovascular system Kexin Medicine Proprotein Convertases Proprotein Convertase 9 Ligation business Intravital microscopy |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017) |
| ISSN: | 2045-2322 |
| Popis: | The effect of lipid lowering on the incidence of deep venous thrombosis (DVT) is controversial. The purpose of this study was to determine the effect of proprotein convertase subtilisin/kexin type 9 (PCSK9) deficiency on development of DVT in mice. Pcsk9 deficient (pcsk9−/−) and wild-type (WT) littermates underwent partial inferior vena cava (IVC) ligation to induce venous thrombosis. 48 hours following IVC ligation, IVC thrombosis was evident in 60% of WT mice and 25% of pcsk9−/− mice (p pcsk9−/− mice. Intravital microscopy performed two hours following partial IVC ligation revealed that leukocyte firm attachment was increased in WT mice compared to mice undergoing a sham operation, however leukocyte attachment was reduced in pcsk9−/− mice compared to WT mice. In conclusion, deficiency of PCSK9 is associated with protection from venous thrombosis. This protection is associated with reduced leukocyte recruitment and NET formation at the site of thrombosis. |
| Databáze: | OpenAIRE |
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